About Alexa Fluor 555

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Alexa Fluor™ 555 (AF555, Alexa 555) has an excitation peak at 555 nm and an emission peak at 565 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC, iFluor® 555 (ATT Bioquest) and iFluor® 560 (ATT Bioquest). Many flow cytometers are equipped with 532nm lasers, so while Alexa 555 can be used for flow cytometry, PE is more commonly used due to its brightness and spectral characteristics. This dye is commonly used for fluorescence microscopy applications.

Alternative Dyes to Alexa Fluor 555

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ATTO 550 CoraLite Plus 555 Cy3

Dyes similar to Alexa Fluor 555 based on emission

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Alexa Fluor 546 ATTO 542 ATTO Rho6G BDY TMR-X SE BODIPY 558/568 BODIPY TMR-X BPE Brilliant Violet 570 Brilliant Ultraviolet 563 Calcein Red AM Calcein Red Calcium Orange CellBrite Fix 555 CellBrite Orange CellMask Orange Plasma Membrane Stain CellROX Orange CellTracker Orange CF532 CF535ST CF540 CF543 CF555 Chromeo 546 CoraLite Plus 555 Cy3 Cyanine 3 SE Cyanine 555 CytoTrack Yellow DiI DY-395XL DY-485XL Dyomics 547 eFluor 565NC eFluor 570 EFLUXX-ID Gold FVS575V HiLyte 555 iFluor 532 iFluor 555 Janelia Fluor 549 LIVE 550 Live/Dead Fix Olive LSSmOrange LysoView 550 MemBrite Fix 543/560 MemBrite Fix 543/560 MITO-ID Green MitoLite Orange FX570 MitoTracker Orange mKO mKusabira-Orange2 mOrange NeuroVue Orange NL 557 NovaFluor Yellow 570 NucView 530 OC515 OFP Oyster 555 PhenoVue 512 Nucleic Acid Stain – complexed with nucleic acids PhenoVue 551 Mitochondrial Stain PhenoVue Fluor 555 PhenoVue ROS-570, Total Oxidative Stress Indicator PKH26 POPO-3 PromoFluor 555P Pyronin Y Qdot 565 ReadiLink 555-570 Rhod-2 (Ca2+ bound) Rhodamine B Seta-555-NHS Spark Blue 574 Spark YG 570 StarBright UltraViolet 575 StarBright Violet 570 SureLight BPE Sytox Orange TFAX 546 SE TMRE ViaKrome 561 VivaFix 547/573 Zombie Yellow NucSpot 555/570 AcidiFluor ORANGE BactoView Dead 560/570 Aluora 555 Lysosome Probe Orange ABflo 555 ABflo 532 mFluor Blue 570 AbBy Fluor 555 XFD546 XFD555 CellTracker CM-Dil BOP-JF549