DY-485XL
DY-485XL (Ex-Max 485 nm/Em-Max 557 nm)
DY-485XL from Dyomics is a member of their MegaStokes fluorophore family. These dyes are characterized by very dramatic Stokes Shifts between their excitation and emission peaks. This fluorophore has an excitation peak at 485 nm and an emission peak at 560 nm. Although spectrally they could be applied to flow cytometry applications, they exhibit wide emission ranges that will spillover into neighboring emission channels. They are most commonly used for fluorescence microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 485 nm
- Emission maximum
- 560 nm
- Optimal laser line
- 488
- Optimal filter
- 575/36
- Relative brightness
- 1 of 5
DY-485XL has an excitation maximum at 485 nm and an emission maximum at 560 nm, a Stokes shift of 75 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is DY-485XL?
- DY-485XL from Dyomics is a member of their MegaStokes fluorophore family. These dyes are characterized by very dramatic Stokes Shifts between their excitation and emission peaks. This fluorophore has an excitation peak at 485 nm and an emission peak at 560 nm. Although spectrally they could be applied to flow cytometry applications, they exhibit wide emission ranges that will spillover into neighboring emission channels. They are most commonly used for fluorescence microscopy.
- What laser excites DY-485XL?
- DY-485XL is optimally excited by the 488 laser line. Its excitation maximum is 485 nm.
- What filter should I use to detect DY-485XL?
- A 575/36 filter captures the peak of the DY-485XL emission near 560 nm with minimal spillover.
- How bright is DY-485XL?
- DY-485XL has a relative brightness of 1 out of 5.