Dyes similar to MemBrite Fix 543/560 based on emissionAlexa Fluor 532 ATTO 542 ATTO Rho6G BDY TMR-X SE BODIPY 558/568 BODIPY TMR-X Brilliant Violet 570 BUV563 Calcein Red AM Calcein Red Calcium Orange CellBrite Fix 555 CellBrite Orange CellMask Orange Plasma Membrane Stain CellROX Orange CellTracker Orange CF532 CF535ST CF540 CF543 CF555 Chromeo 546 CMA-Chromomycin A3 CoraLite 532 CoraLite 555 Cy3 Cyanine 3 SE Cyanine 555 CytoTrack Yellow DiI DY-395XL DY-485XL eFluor 565NC eFluor 570 EFLUXX-ID Gold FVS575V HiLyte 555 iFluor 514 iFluor 532 iFluor 555 Janelia Fluor 549 Live/Dead Fix Olive LSSmOrange LysoBrite Orange LysoView 550 mHoneyDew MITO-ID Green MitoLite Orange FX570 mKO mKusabira-Orange2 mOrange NeuroVue Orange NovaFluor Blue 555 NovaFluor Yellow 570 Nuclear Orange NucView 530 OC515 OFP Oyster 550 PhenoVue 551 Mitochondrial Stain PhenoVue Fluor 555 PKH26 POPO-3 PromoFluor 555P Pyronin Y Qdot 565 ReadiLink 555-570 Rhodamine B Seta-555-NHS Spark Blue 574 Spark YG 570 StarBright UltraViolet 575 StarBright Violet 570 SureLight BPE Sytox Orange TFAX 546 SE ViaKrome 561 Zombie Yellow
About MemBrite Fix 543/560
Biotium’s MemBrite Fix and Membrite Fix-ST dyes are cell surface staining dyes that irreversibly react with cell surface proteins to stain the outer membrane. A great benefit to these dyes is that MemBrite Fix dyes can withstand fixation and permeabilization which allows extracellular staining to be combined with intracellular staining techniques. Additionally, the MemBrite Fix-ST dyes (and MemBrite Fix 680/700) are compatible with super resolution microscopy and imaging techniques such as STORM. This dye line shares similarities with Biotium’s CellBrite line, but there are a few key differences. CellBrite Fix stains are easy to use and require a single staining step while MemBrite Fix dyes require a two-step staining protocol. While ease of use is important, MemBrite Fix and Membrite Fix-ST dyes have more colors to choose from and they do not associate with lipids in the membrane which leads to lower cytoplasmic background after permeabilization.