MemBrite Fix 543/560
MemBrite Fix 543/560 (Ex-Max 543 nm/Em-Max 560 nm)
Biotium's MemBrite Fix and Membrite Fix-ST dyes are amine-reactive cell surface staining dyes that conjugate cell surface proteins to stain the outer membrane of live cells. A great benefit to these dyes is that MemBrite Fix dyes can withstand fixation and permeabilization which allows extracellular staining to be combined with intracellular staining techniques. Additionally, the MemBrite Fix-ST dyes (and MemBrite Fix 680/700) are compatible with super resolution microscopy and Fluorescence Microscopy techniques such as STORM. MemBrite Fix 543/560 has an excitation peak at 543 nm and an emission peak at 560 nm and is recommended for use in fluorescence microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 543 nm
- Emission maximum
- 560 nm
- Optimal laser line
- 532
- Commercial antibodies
- 2 from 1 supplier
MemBrite Fix 543/560 has an excitation maximum at 543 nm and an emission maximum at 560 nm, a Stokes shift of 17 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is MemBrite Fix 543/560?
- Biotium's MemBrite Fix and Membrite Fix-ST dyes are amine-reactive cell surface staining dyes that conjugate cell surface proteins to stain the outer membrane of live cells. A great benefit to these dyes is that MemBrite Fix dyes can withstand fixation and permeabilization which allows extracellular staining to be combined with intracellular staining techniques. Additionally, the MemBrite Fix-ST dyes (and MemBrite Fix 680/700) are compatible with super resolution microscopy and Fluorescence Microscopy techniques such as STORM. MemBrite Fix 543/560 has an excitation peak at 543 nm and an emission peak at 560 nm and is recommended for use in fluorescence microscopy.
- What laser excites MemBrite Fix 543/560?
- MemBrite Fix 543/560 is optimally excited by the 532 laser line. Its excitation maximum is 543 nm.
- How many antibodies are available conjugated to MemBrite Fix 543/560?
- FluoroFinder tracks 2 commercially available MemBrite Fix 543/560 conjugates, searchable by target, clone, host and application.