Qdot 565 Dye Profile
Dyes similar to Qdot 565 based on emissionAlexa Fluor 546 ATTO 542 ATTO Rho6G BDY TMR-X SE BODIPY 558/568 BODIPY TMR-X BPE Brilliant Violet 570 BUV563 Calcein Red AM Calcein Red Calcium Orange CellBrite Fix 555 CellBrite Orange CellMask Orange Plasma Membrane Stain CellROX Orange CellTracker Orange CF532 CF535ST CF540 CF543 CF555 Chromeo 546 CMA-Chromomycin A3 CoraLite Plus 555 Cy3 Cyanine 3 SE Cyanine 555 CytoTrack Yellow DiI DY-395XL DY-485XL Dyomics 547 eFluor 565NC eFluor 570 EFLUXX-ID Gold FVS575V HiLyte 555 iFluor 532 iFluor 555 Janelia Fluor 549 Live/Dead Fix Olive LSSmOrange LysoView 550 MemBrite Fix 543/560 MITO-ID Green MitoLite Orange FX570 MitoTracker Orange mKO mKusabira-Orange2 mOrange NeuroVue Orange NL 557 NovaFluor Yellow 570 NucView 530 OC515 OFP Oyster 550 PhenoVue 551 Mitochondrial Stain PhenoVue Fluor 555 PKH26 POPO-3 PromoFluor 555P Pyronin Y ReadiLink 555-570 Rhod-2 (Ca2+ bound) Rhodamine B Seta-555-NHS Spark Blue 574 Spark YG 570 StarBright UltraViolet 575 StarBright Violet 570 SureLight BPE Sytox Orange TFAX 546 SE TMRE ViaKrome 561 VivaFix 547/573 Zombie Yellow
About Qdot 565
Qdot™ 565 is a yellow-orange-emitting nanocrystal based fluorochrome that can be excited by lasers from 350 nm to 525 nm and collected using any filter designed to capture light from TRITC, such as a 585/42 bandpass filter. Qdot™ 565 has an excitation maximum of around 300 nm and an emission maximum of 565 nm. Due to its unique excitation spectrum, not many dyes have identical excitation and emission spectrums, but the nearest alternatives include Brilliant Violet™ 570, TRITC, and eFluor 565NC. Qdot™ 565 exhibits a very high level of brightness and high photostability in buffer. It is compatible with many applications, but it works particularly well for imaging, flow cytometry, and other applications to detect low abundance targets or with multiplexing. This dye is part of the Qdot™ line of fluorochromes that all have similar excitation curves, with single sharp emission peaks that make multiplexing these dyes while exciting with one laser easy.