CF543
CF®543 (Ex-Max 541 nm/Em-Max 560 nm)
CF®543 from Biotium is a fluorophore with an excitation peak at 541 nm and an emission peak at 560 nm. It can be useful in both fluorescence microscopy and flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 541 nm
- Emission maximum
- 560 nm
- Optimal laser line
- 532
- Optimal filter
- 585/42
- Relative brightness
- 2 of 5
- Extinction coefficient
- 100000
- Molecular weight
- 870
- Commercial antibodies
- 44 from 1 supplier
CF543 has an excitation maximum at 541 nm and an emission maximum at 560 nm, a Stokes shift of 19 nm. Its extinction coefficient is 100000 M⁻¹cm⁻¹. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is CF543?
- CF®543 from Biotium is a fluorophore with an excitation peak at 541 nm and an emission peak at 560 nm. It can be useful in both fluorescence microscopy and flow cytometry.
- What laser excites CF543?
- CF543 is optimally excited by the 532 laser line. Its excitation maximum is 541 nm.
- What filter should I use to detect CF543?
- A 585/42 filter captures the peak of the CF543 emission near 560 nm with minimal spillover.
- How bright is CF543?
- CF543 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to CF543?
- FluoroFinder tracks 44 commercially available CF543 conjugates, searchable by target, clone, host and application.