SiRA 2
SiRA 2 has an excitation peak at 649 nm and an emission peak at 662 nm. SiRA 2 (silicon rhodamine 2) is a near-infrared (NIR) fluorogen for SiRA light-up aptamer. SiRA 2 enables imaging of single RNA in living cells. SiRA 2 is extremely bright, photostable and allows the visualization of mRNA expression in bacteria in no-wash, quencher-free, live-cell imaging experiments. Cell permeable.
Excitation & emission spectra
Key facts
- Excitation maximum
- 649 nm
- Emission maximum
- 662 nm
- Optimal laser line
- 640
- Extinction coefficient
- 86000
- Quantum yield
- 0.98
- Molecular weight
- 602.81
SiRA 2 has an excitation maximum at 649 nm and an emission maximum at 662 nm, a Stokes shift of 13 nm. Its extinction coefficient is 86000 M⁻¹cm⁻¹. It is optimally excited by the 640 laser line.
Frequently asked questions
- What is SiRA 2?
- SiRA 2 has an excitation peak at 649 nm and an emission peak at 662 nm. SiRA 2 (silicon rhodamine 2) is a near-infrared (NIR) fluorogen for SiRA light-up aptamer. SiRA 2 enables imaging of single RNA in living cells. SiRA 2 is extremely bright, photostable and allows the visualization of mRNA expression in bacteria in no-wash, quencher-free, live-cell imaging experiments. Cell permeable.
- What laser excites SiRA 2?
- SiRA 2 is optimally excited by the 640 laser line. Its excitation maximum is 649 nm.