iRFP 670
iRFP 670 (Ex-Max 643 nm/Em-Max 670 nm)
iRFP670 is a dimeric fluorescent protein derived from the proteobacteria, Rhodopseudomonas palustris. It has an excitation peak at 643 nm and an emission peak at 670 nm. It requires the cofactor biliverdin for fluorescence.
Excitation & emission spectra
Key facts
- Excitation maximum
- 643 nm
- Emission maximum
- 670 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Extinction coefficient
- 114000
- Quantum yield
- 0.11
- Molecular weight
- 34500
iRFP 670 has an excitation maximum at 643 nm and an emission maximum at 670 nm, a Stokes shift of 27 nm. Its extinction coefficient is 114000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is iRFP 670?
- iRFP670 is a dimeric fluorescent protein derived from the proteobacteria, Rhodopseudomonas palustris. It has an excitation peak at 643 nm and an emission peak at 670 nm. It requires the cofactor biliverdin for fluorescence.
- What laser excites iRFP 670?
- iRFP 670 is optimally excited by the 628-640 laser line. Its excitation maximum is 643 nm.
- What filter should I use to detect iRFP 670?
- A 660/20 filter captures the peak of the iRFP 670 emission near 670 nm with minimal spillover.