NucSpot 640
NucSpot 640 (Ex-Max 649 nm/Em-Max 668 nm)
NucSpot 650/665 is a cell-impermeant, fluorogenic nucleic acid stain used live/dead discrimination or counterstaining fixed-cells. It has an excitation peak at 649 nm and an emission peak at 668 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 649 nm
- Emission maximum
- 668 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
NucSpot 640 has an excitation maximum at 649 nm and an emission maximum at 668 nm, a Stokes shift of 19 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is NucSpot 640?
- NucSpot 650/665 is a cell-impermeant, fluorogenic nucleic acid stain used live/dead discrimination or counterstaining fixed-cells. It has an excitation peak at 649 nm and an emission peak at 668 nm.
- What laser excites NucSpot 640?
- NucSpot 640 is optimally excited by the 628-640 laser line. Its excitation maximum is 649 nm.
- What filter should I use to detect NucSpot 640?
- A 660/20 filter captures the peak of the NucSpot 640 emission near 668 nm with minimal spillover.