Live-or-Dye 640/662
Live-or-Dye™ 640/662 (Ex-Max 642 nm/Em-Max 633 nm)
Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 640/662 has an excitation peak at 640 nm and an emission peak at 662 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 642 nm
- Emission maximum
- 662 nm
- Optimal laser line
- 628-640
- Optimal filter
- 630/69
- Commercial conjugates
- 2 from 1 supplier
Live-or-Dye 640/662 has an excitation maximum at 642 nm and an emission maximum at 662 nm, a Stokes shift of 20 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Live-or-Dye 640/662?
- Biotium's Live-or-Dye™ dyes are amine-reactive and used for live/dead cell discrimination in flow cytometry. These probes pass through the permeable cell membrane of dead cells to stain intracellular amine groups. However, the probe cannot pass through the intact membranes of live cells. Additionally, these dyes are very stable and can withstand fixation, permeabilization, and will not transfer between cells. Live-or-Dye™ 640/662 has an excitation peak at 640 nm and an emission peak at 662 nm.
- What laser excites Live-or-Dye 640/662?
- Live-or-Dye 640/662 is optimally excited by the 628-640 laser line. Its excitation maximum is 642 nm.
- What filter should I use to detect Live-or-Dye 640/662?
- A 630/69 filter captures the peak of the Live-or-Dye 640/662 emission near 662 nm with minimal spillover.
- How many antibodies are available conjugated to Live-or-Dye 640/662?
- FluoroFinder tracks 2 commercially available Live-or-Dye 640/662 conjugates, searchable by target, clone, host and application.