APC Dye Profile
640

660/20

651

660

About APC
Allophycocyanin (APC) is a red-emitting fluorescent protein that can be excited by the 594 nm orange or 640 nm red lasers and captured with a 660/20 nm bandpass filter. This dye is a large organic molecule derived from red algae and like PE (phycoerythrin), it is of the phycobilin protein family, known for water solubility among other advantageous characteristics. APC has an excitation peak at 650 nm and a emission peak at 660 nm, and is spectrally similar to Alexa Fluor® 647 (ThermoFisher Scientific), DyLight™ 650 (ThermoFisher Scientific), eFluor™ 660 (ThermoFisher Scientific), Cy®5 (GE Healthcare), and iFluor™ 647 (ATT Bioquest). APC is very bright and is commony used in many applications. It is also commonly used as the donor molecule for tandem fluorophores. APC is a very good donor molecule for tandem fluorophores and comes in many tandem varieties. This dye is a generic with no sole manufacturer or trademark, though that is not the case for all tandem varieties.
Spectra
Dyes similar to APC based on emission
Check to add to spectra viewerAlexa Fluor 647 ATTO 633 ATTO 647 ATTO 647N Brilliant Blue 660-P2 BDY 650-X SE BODIPY 650/665 Brilliant Ultraviolet 661 Calcein Deep Red AM Calcein Deep Red CellBrite Fix 640 CellROX Deep Red CellTrace Far Red CellVue Maroon CF640R CF647 CF647ST Chromeo 642 CoraLite Plus 647 Cy5 Cyanine 5 SE Cyanine 647 CypHer5E DiD DY-520XL DY-521XL DyLight 633 eFluor 660 eFluor 660 Fix Viability eVolve 655 ExoBrite 640/660 EV Membrane Stain Flash Phalloidin NIR 647 Fura Red AM (Ca2+free) FVS660 FxCycle Far Red Helix NP NIR iFluor 633 IRDye 650 Janelia Fluor 635 Janelia Fluor 646 LipidSpot 610 LysoView 633 MagDot 655 MemBrite Fix 640/660 MemBrite Fix ST 650/665 MitoLite Deep Red FX660 MultiDot 655 NeuroVue Maroon Nile Blue NirFP NL 637 NovaFluor Blue 660 / 120S NovaFluor Blue 660 / 40S NovaFluor Red 660 NovaFluor Yellow 660 NucSpot 640 NucSpot Far-Red SureLight P3 PE-Alexa 647 PE-Cy5 PhenoVue 641 Mitochondrial Stain PromoFluor 633P Qdot 655 ReadiLink 633-655 ROS-ID Red STAR 635 STAR 635P StarBright UltraViolet 665 STAR RED SureLight APC SureLight PE Cyanine 5.0 Sytox Red TagRFP657 Thiazole Red Thiazole Red Homodimer TO-Pro 3 TOTO-3 ViaKrome 638 Vio667 VioBright 667 VivaFix 649/660 Vybrant DyeCycle Orange
Popular APC Antibodies
Company/SKU | Applications | Marker / Reactivity | Host / Isotype / Clone | Size / Price | Details |
---|---|---|---|---|---|
R&D Systems IC5868A |
ICS | Arginase 1 Human,Mouse |
Sheep IgG |
100 Tests $431 |
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Miltenyi Biotec 130-113-563 |
MICS (MACSima Imaging Cyclic Staining),IF,FC,IHC | CD159a (NKG2A) Human |
Human IgG1 REA110 |
100 tests in 200 µL $320 |
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Miltenyi Biotec 130-110-520 |
MICS (MACSima Imaging Cyclic Staining),IF,FC,IHC | CD14 Human |
Human IgG1 REA599 |
100 tests in 200 µL $325 |
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Miltenyi Biotec 130-111-601 |
MICS (MACSima Imaging Cyclic Staining),IF,FC,IHC | FoxP3 Mouse |
Human IgG1 REA788 |
150 µg in 1 mL $365 |
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R&D Systems FAB5538A-100 |
FC | CD192 Mouse |
Rat IgG2b 475301 |
100 Tests $395 |
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Miltenyi Biotec 130-097-371 |
FC | CLEC9A Human |
Mouse IgG2a 8F9 |
100 Tests in 1 ml $325 |
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Miltenyi Biotec 130-113-135 |
MICS (MACSima Imaging Cyclic Staining),IF,FC,IHC | CD3 Human |
Human IgG1 REA613 |
100 tests in 200 µL $320 |
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R&D Systems FAB17291A |
FC | TREM-2 Human,Mouse |
Rat IgG2b 237920 |
100 Tests $431 |
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MBL International TB-M507-2 |
FC | p15E KSPWFTTL (Tetramer) Mouse |
Mouse Tetramer H-2Kb |
50 Tests $1,350 |
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Miltenyi Biotec 130-113-231 |
MICS (MACSima Imaging Cyclic Staining),IF,FC,IHC | CD11b Human,Mouse |
Rat IgG2b M1/70.15.11.5 |
100 tests in 200 µL $320 |
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Popular APC Secondary Antibodies
Company/SKU | Applications | Marker / Reactivity | Host / Isotype / Clone | Size / Price | Details |
---|---|---|---|---|---|
BioLegend 405714 |
FC | IgD Mouse |
Rat IgG2a κ 11-26c.2a |
100 μg $261 |
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BioLegend 406509 |
FC | IgM Mouse |
Rat IgG2a κ RMM-1 |
100 μg $296 |
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BD Biosciences 551062 |
FC | IgM Human |
Mouse IgG1 κ G20-127 |
100 Tests $310 |
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BioLegend 405713 |
FC | IgD Mouse |
Rat IgG2a κ 11-26c.2a |
25 μg $102 |
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R&D Systems F0111 |
FC | IgG Rabbit |
Goat IgG |
100 Tests $265 |
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BD Biosciences 550676 |
FC | IgM Mouse |
Rat IgG2a κ II/41 |
100 μg $295 |
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BD Biosciences 561303 |
FC | IgD Human |
Mouse IgG2a κ IA6-2 |
50 Tests $185 |
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BD Biosciences 560868 |
FC | IgD Mouse |
Rat IgG2a κ 11-26c.2a |
50 μg $130 |
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BioLegend 348222 |
FC | IgD Human |
Mouse IgG2a κ IA6-2 |
100 Tests $296 |
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BioLegend 405308 |
FC | IgG Mouse |
Goat IgG Poly4053 |
100 μg $237 |
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APC Conjugation Kits
Product Type | Company/SKU | Applications | Size / Price | Details |
---|---|---|---|---|
Conjugation Kit |
Bio-Rad LNK031APC |
Labeling | 1 Conjμgation for 100μg antibody $265 |
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Conjugation Kit |
Expedeon 705-0010 |
Labeling | 3 x 100 μg $428 |
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Conjugation Kit |
Expedeon 705-0030 |
Labeling | 3 x 10 μg $252 |
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Conjugation Kit |
Expedeon 705-0005 |
Labeling | 100 μg $254 |
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Conjugation Kit |
705-0030 |
Labeling | 3 x 0.01 mg $299 |
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Conjugation Kit |
Expedeon 705-0015 |
Labeling | 1 x 1 mg $634 |
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Conjugation Kit |
Bio-Rad LNK033APC |
Labeling | 1 Conjμgation for 1mg antibody $735 |
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Conjugation Kit |
Bio-Rad LNK032APC |
Labeling | 3 Conjμgations for 100μg antibody $398 |
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Conjugation Kit |
Bio-Rad LNK034APC |
Labeling | 3 Conjμgations for 10μg antibody $265 |
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Conjugation Kit |
Biotium 92306 |
Labeling | 25-50 μg $122 |
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Conjugation Kit |
Biotium 92307 |
Labeling | 50-100 μg $133 |
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