About APC

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Allophycocyanin (APC) is a red-emitting fluorescent protein that can be excited by the 594 nm orange or 640 nm red lasers and captured with a 660/20 nm bandpass filter. This dye is a large organic molecule derived from red algae and like PE (phycoerythrin), it is of the phycobilin protein family, known for water solubility among other advantageous characteristics. APC has an excitation peak at 650 nm and a emission peak at 660 nm, and is spectrally similar to Alexa Fluor® 647 (ThermoFisher Scientific), DyLight™ 650 (ThermoFisher Scientific), eFluor™ 660 (ThermoFisher Scientific), Cy®5 (GE Healthcare), and iFluor™ 647 (ATT Bioquest). APC is very bright and is commony used in many applications. It is also commonly used as the donor molecule for tandem fluorophores. APC is a very good donor molecule for tandem fluorophores and comes in many tandem varieties. This dye is a generic with no sole manufacturer or trademark, though that is not the case for all tandem varieties.

Dyes similar to APC based on emission

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Alexa Fluor 647 ATTO 633 ATTO 647 ATTO 647N Brilliant Blue 660-P2 BDY 650-X SE BODIPY 650/665 Brilliant Ultraviolet 661 Calcein Deep Red AM Calcein Deep Red CellBrite Fix 640 CellROX Deep Red CellTrace Far Red CellVue Maroon CF640R CF647 CF647ST Chromeo 642 CoraLite Plus 647 Cy5 Cyanine 5 SE Cyanine 647 CypHer5E DiD DY-520XL DY-521XL DyLight 633 eFluor 660 eFluor 660 Fix Viability eVolve 655 ExoBrite 640/660 EV Membrane Stain Flash Phalloidin NIR 647 Fura Red AM (Ca2+free) FVS660 FxCycle Far Red Helix NP NIR iFluor 633 IRDye 650 Janelia Fluor 635 Janelia Fluor 646 LipidSpot 610 LysoView 633 MagDot 655 MemBrite Fix 640/660 MemBrite Fix ST 650/665 MitoLite Deep Red FX660 MultiDot 655 NeuroVue Maroon Nile Blue NirFP NL 637 NovaFluor Blue 660 / 120S NovaFluor Blue 660 / 40S NovaFluor Red 660 NovaFluor Yellow 660 NucSpot 640 NucSpot Far-Red SureLight P3 PE-Alexa 647 PE-Cy5 PhenoVue 641 Mitochondrial Stain PromoFluor 633P Qdot 655 ReadiLink 633-655 ROS-ID Red STAR 635 STAR 635P StarBright UltraViolet 665 STAR RED SureLight APC SureLight PE Cyanine 5.0 Sytox Red TagRFP657 Thiazole Red Thiazole Red Homodimer TO-Pro 3 TOTO-3 ViaKrome 638 Vio667 VioBright 667 VivaFix 649/660 Vybrant DyeCycle Orange