Alexa Fluor 647 Dye Profile
640

660/20

650

668

About Alexa Fluor 647
Alexa Fluor® 647 (AF647, Alexa 647) is a red-emitting synthetic fluorophore that can be excited by the 640 nm red laser and captured with a 660/20 nm bandpass filter. AF647 has an excitation peak at 650 nm and an emission peak at 668 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor™ 647 (ATT Bioquest), APC and DyLight™ 650 (ThermoFisher Scientific). AF647 is commonly used for flow cytometry, microscopy, super-resolution microscopy as well as imaging applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye. The Alexa Fluor dye family was originally developed by Molecular Probes, now a part of ThermoFisher Scientific. AlexaFluor is a registered trademark of Invitrogen, ThermoFisher Scientific. Alexa Fluor dyes come in a wide variety of modified formats that allow for easy conjugations or right out of the box use.
Spectra
Dyes similar to Alexa Fluor 647 based on emission
Check to add to spectra viewerAPC ATTO 647 ATTO 647N BactoView Live Red Brilliant Blue 660-P2 BDY 650-X SE BODIPY 650/665 Brilliant Ultraviolet 661 Calcein Deep Red AM CellBrite Fix 640 CellBrite Red CellBrite Steady 650 CellMask Deep Red Plasma Membrane Stain CellROX Deep Red CellTrace Far Red CellVue Maroon CellVue Plum CF640R CF647 CF647ST CF650 Chromeo 642 CoraLite Plus 647 CPN680 Red Cy5 Cyanine 5 SE Cyanine 647 CypHer5E DiD DY-520XL DY-521XL DyLight 649 DyLight 650 Dyomics 647 eFluor 660 eFluor 670 Cell Proliferation ER-ID Red ExoBrite 640/660 EV Membrane Stain Flash Phalloidin NIR 647 FluoProbes647H Fura Red AM (Ca2+free) FVS660 HiLyte 647 iFluor 647 IRDye 650 iRFP 670 Janelia Fluor 646 LipidSpot 610 Live/Dead Fix Far Red LysoView 633 LysoView 640 MaxLight 650 MemBrite Fix 640/660 MemBrite Fix ST 650/665 MitoView 650 NeuroVue Maroon Nile Blue NirFP NovaFluor Blue 660 / 120S NovaFluor Blue 660 / 40S NovaFluor Red 660 NovaFluor Yellow 660 NucSpot 640 NucSpot Far-Red Oyster 650 SureLight P3 PE-Alexa 647 PE-Cy5 PE-Dyomics 647 PerCP PhenoVue 641 Mitochondrial Stain PromoFluor 647P ReadiLink 647-674 ROS-ID Red RPE-CF647T Seta-650-NHS Seta-PerCP-680 Seta-R-PE-670 StarBright UltraViolet 665 SureLight APC SureLight PE Cyanine 5.0 SureLight PerCP Thiazole Red Homodimer TO-Pro 3 TOTO-3 Tri-Color Vio667 VioBright 667 VivaFix 649/660
Popular Alexa Fluor 647 Antibodies
Company/SKU | Applications | Marker / Reactivity | Host / Isotype / Clone | Size / Price | Details |
---|---|---|---|---|---|
R&D Systems FAB25381R-100UG |
FC | DDR2 Human |
Mouse IgG2b 290804 |
100 μg $319 |
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Sony 3667040 |
IF,FC | H2A.X-Phospho (Ser139) Human,Mouse |
Mouse IgG1 κ 2F3 |
100 Tests $365 |
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Novus Biologicals NB600-344AF647 |
ICC, IF,WB | Flag Tag Monkey,All Species,Human,Primate |
Goat IgG |
0.1 ml $464 |
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Novus Biologicals NBP1-04676AF647 |
ICS, FC,Knockout Validated,ICC, IF,FC,IHC-P,IHC-Fr,WB,IHC | PGC-1 alpha Human,Rat,Mouse,Squirrel,Hamster,Porcine,Goat |
Rabbit IgG |
0.1 ml $518 |
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R&D Systems FAB3478R-100UG |
FC | CCR10 Human |
Rat IgG2a 314305 |
100 μg $405 |
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R&D Systems FAB173R-100UG |
FC | CXCR4 Human |
Mouse IgG2b 44717 |
100 μg $362 |
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R&D Systems FAB17291R-025 |
FC | TREM-2 Human,Mouse |
Rat IgG2b 237920 |
25 Tests $205 |
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R&D Systems FAB9927R-100UG |
FC | CD25 Human |
Human IgG1 Hu102 |
100 μg $384 |
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Sony 2201065 |
FC | FoxP3 Human,Cynomolgus,Rhesus,Baboon,Chimpanzee |
Mouse IgG1 κ 259D |
25 Tests $162 |
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R&D Systems FAB1125R-100UG |
FC | TER-119 Mouse |
Rat IgG2b TER-119 |
100 μg $388 |
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Popular Alexa Fluor 647 Secondary Antibodies
Company/SKU | Applications | Marker / Reactivity | Host / Isotype / Clone | Size / Price | Details |
---|---|---|---|---|---|
BioLegend 405707 |
3D-IHC,FC | IgD Mouse |
Rat IgG2a κ 11-26c.2a |
25 μg $112 |
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BioLegend 405416 |
FC,IHC-Fr | IgG Rat |
Goat IgG Poly4054 |
100 μg $188 |
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BioLegend 410714 |
FC | IgG Human |
Rat IgG2a κ M1310G05 |
100 Tests $347 |
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BioLegend 407116 |
FC | IgG2a Mouse |
Rat IgG RMG2a-62 |
100 μg $194 |
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BioLegend 348228 |
FC | IgD Human |
Mouse IgG2a κ IA6-2 |
100 Tests $358 |
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BioLegend 405708 |
3D-IHC,FC | IgD Mouse |
Rat IgG2a κ 11-26c.2a |
100 μg $265 |
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BioLegend 406525 |
FC | IgM Mouse |
Rat IgG2a κ RMM-1 |
25 μg $89 |
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BioLegend 405322 |
FC,IHC-P | IgG Mouse |
Goat IgG Poly4053 |
100 μg $183 |
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Thermo Fisher Scientific A21238 |
IF,FC | IgM Mouse |
Goat IgG Polyclonal |
250 μl $139 |
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Thermo Fisher Scientific A21246 |
IF,FC | IgG Rabbit |
Goat IgG Polyclonal |
250 μl $139 |
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Alexa Fluor 647 Conjugation Kits
Product Type | Company/SKU | Applications | Size / Price | Details |
---|---|---|---|---|
Conjugation Kit |
Thermo Fisher Scientific A20186 |
Labeling | 5 x 100 μg $484 |
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