About Alexa Fluor 647

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Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye.

Alternative Dyes to Alexa Fluor 647

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ATTO 647 ATTO 647N Cy5

Dyes similar to Alexa Fluor 647 based on emission

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APC ATTO 633 ATTO 647 ATTO 647N Brilliant Blue 660-P2 BDY 650-X SE BODIPY 650/665 Brilliant Ultraviolet 661 Calcein Deep Red AM CellBrite Fix 640 CellBrite Red CellROX Deep Red CellTrace Far Red CellVue Maroon CellVue Plum CF640R CF647 CF647ST CF650 Chromeo 642 CoraLite Plus 647 Cy5 Cyanine 5 SE Cyanine 647 CypHer5E DiD DY-481XL DY-520XL DY-521XL DyLight 633 DyLight 649 DyLight 650 Dyomics 647 eFluor 660 eFluor 660 Fix Viability eFluor 670 Cell Proliferation ExoBrite 640/660 EV Membrane Stain Flash Phalloidin NIR 647 FluoroProbes647h FLUX 640 FLUX 647 Fura Red AM (Ca2+bound) Fura Red AM (Ca2+free) FVS660 FxCycle Far Red iFluor 647 IRDye 650 iRFP 670 Janelia Fluor 646 LipidSpot 610 Live/Dead Fix Far Red Live-or-Dye 640/662 LysoView 633 LysoView 640 MemBrite Fix 640/660 MemBrite Fix ST 650/665 MitoLite Deep Red FX660 MitoView 650 NeuroVue Maroon Nile Blue NirFP NL 637 NovaFluor Blue 660 / 120S NovaFluor Blue 660 / 40S NovaFluor Red 660 NovaFluor Yellow 660 NucSpot 640 NucSpot Far-Red Oyster 650 SureLight P3 PE-Alexa 647 PE-Cy5 PE-Dyomics 647 PhenoVue 641 Mitochondrial Stain PhenoVue Fluor 647 PhenoVue Fluor 647 Live Cell PhenoVue ROS-670, Total Oxidative Stress Indicator PromoFluor 633P PromoFluor 647P ReadiLink 647-674 ROS-ID Red RPE-CF647T Seta-650-NHS Seta-R-PE-670 STAR 635 StarBright UltraViolet 665 StarBright Violet 670 StarBright Yellow 665 STAR RED SureLight APC SureLight PE Cyanine 5.0 Thiazole Red Thiazole Red Homodimer TO-Pro 3 TOTO-3 Tri-Color TSA Vivid 650 Vio 667 Vio Bright 667 VivaFix 649/660 CoraLite 647 Thalidomide-Cyanine 5 SiRA 2 Janelia Fluor 646b, Maleimide RayBright Red 647 cFluor R668 CytoLiner 650/675 RayBright APC Janelia Fluor 630b, Maleimide Janelia Fluor 630b, NHS ester Janelia Fluor 635, Tetrazine ABflo 647 Aluora 647 ExoBrite 645/675 True EV Membrane Stain ExoBrite 655/670 Janelia Fluor 635b, Maleimide AbBy Fluor 647 ExoBrite 640/660 Janelia Fluor 635b, NHS ester Alexa Fluor 647-RPE HB-2-30 NucSpot 650/665 XFD647 RayBright LIVE R647 ATTO 643 JFX 650, NHS ester RealBlue 670 Janelia Fluor 646, Haloalkane JFX 650, Maleimide BactoView Dead 655/670