Alexa Fluor 647 Dye Profile

Thermo Fisher Scientific™

628-640

Laser Line

660/20

Common Filter

650

Excitation Max

665

Emission Max

239000

Extinction Coefficient

0.33

Quantun Efficiency
()

800

Molecular Weight

About Alexa Fluor 647


Alexa Fluor™ 647 (AF647, Alexa 647) has an excitation peak at 650 nm and an emission peak at 665 nm, and is spectrally similar to Cy®5 (GE Healthcare), iFluor® 647 (ATT Bioquest), and DyLight™ 650 (Thermo Fisher Scientific). Alexa 647 is commonly used for flow cytometry, microscopy, super-resolution microscopy applications. It is very bright, photostable, and pH insensitive, all of which contribute to sensitive detection while using this dye.



Spectra


Alternative Dyes to Alexa Fluor 647

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ATTO 647 Cy5 ATTO 647N


Dyes similar to Alexa Fluor 647 based on emission

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APC ATTO 633 ATTO 647 ATTO 647N Brilliant Blue 660-P2 BDY 650-X SE BODIPY 650/665 Brilliant Ultraviolet 661 Calcein Deep Red AM CellBrite Fix 640 CellBrite Red CellROX Deep Red CellTrace Far Red CellVue Maroon CellVue Plum CF640R CF647 CF647ST CF650 Chromeo 642 CoraLite Plus 647 Cy5 Cyanine 5 SE Cyanine 647 CypHer5E DiD DY-481XL DY-520XL DY-521XL DyLight 633 DyLight 649 DyLight 650 Dyomics 647 eFluor 660 eFluor 660 Fix Viability eFluor 670 Cell Proliferation ExoBrite 640/660 EV Membrane Stain Flash Phalloidin NIR 647 FluoProbes647H FLUX 640 FLUX 647 Fura Red AM (Ca2+bound) Fura Red AM (Ca2+free) FVS660 FxCycle Far Red iFluor 647 IRDye 650 iRFP 670 Janelia Fluor 646 LipidSpot 610 Live/Dead Fix Far Red Live-or-Dye 640/662 LysoView 633 LysoView 640 MemBrite Fix 640/660 MemBrite Fix ST 650/665 MitoLite Deep Red FX660 MitoView 650 NeuroVue Maroon Nile Blue NirFP NL 637 NovaFluor Blue 660 / 120S NovaFluor Blue 660 / 40S NovaFluor Red 660 NovaFluor Yellow 660 NucSpot 640 NucSpot Far-Red Oyster 650 SureLight P3 PE-Alexa 647 PE-Cy5 PE-Dyomics 647 PhenoVue 641 Mitochondrial Stain PhenoVue Fluor 647 PhenoVue Fluor 647 Live Cell PhenoVue ROS-670, Total Oxidative Stress Indicator PromoFluor 633P PromoFluor 647P ReadiLink 647-674 ROS-ID Red RPE-CF647T Seta-650-NHS Seta-R-PE-670 STAR 635 StarBright UltraViolet 665 StarBright Violet 670 StarBright Yellow 665 STAR RED SureLight APC SureLight PE Cyanine 5.0 Thiazole Red Thiazole Red Homodimer TO-Pro 3 TOTO-3 Tri-Color TSA Vivid 650 Vio667 VioBright 667 VivaFix 649/660

Popular Alexa Fluor 647 Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
R&D Systems
FAB25381R-100UG
FC DDR2
Human
Mouse
IgG2b
290804
100 μg
$319
View
Sony
4032060
FC STAT1 (pS727)
Human,Mouse
Mouse
IgG1 κ
A15158B
100 Tests
$329
View
Sony
3667040
FC,IF H2A.X-Phospho (Ser139)
Human,Mouse
Mouse
IgG1 κ
2F3
100 Tests
$365
View
Novus Biologicals
NB600-344AF647
ICC, IF,WB Flag Tag
Monkey,All Species,Human,Primate
Goat
IgG
0.1 ml
$464
View
Novus Biologicals
NBP1-04676AF647
FC,ICS, FC,Knockout Validated,ICC, IF,IHC-P,WB,IHC-Fr,IHC PGC-1 alpha
Human,Rat,Mouse,Squirrel,Hamster,Porcine,Goat
Rabbit
IgG
0.1 ml
$518
View
R&D Systems
FAB3478R-100UG
FC CCR10
Human
Rat
IgG2a
314305
100 μg
$405
View
R&D Systems
FAB17291R-025
FC TREM-2
Human,Mouse
Rat
IgG2b
237920
25 Tests
$205
View
R&D Systems
FAB3715R-100UG
FC Fibroblast Activation Protein alpha
Human
Mouse
IgG1
427819
100 μg
$373
View
R&D Systems
FAB173R-100UG
FC CXCR4
Human
Mouse
IgG2b
44717
100 μg
$362
View
R&D Systems
FAB4867R-100
FC CD19
Human
Mouse
IgG1
4G7-2E3
100 Tests
$215
View
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Popular Alexa Fluor 647 Secondary Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
BioLegend
410714
FC IgG
Human
Rat
IgG2a κ
M1310G05
100 Tests
$347
View
BioLegend
405707
FC,3D-IHC IgD
Mouse
Rat
IgG2a κ
11-26c.2a
25 μg
$112
View
BioLegend
405416
FC,IHC-Fr IgG
Rat
Goat
IgG
Poly4054
100 μg
$188
View
BioLegend
405708
FC,3D-IHC IgD
Mouse
Rat
IgG2a κ
11-26c.2a
100 μg
$265
View
BioLegend
348228
FC IgD
Human
Mouse
IgG2a κ
IA6-2
100 Tests
$358
View
BioLegend
405322
FC,IHC-P IgG
Mouse
Goat
IgG
Poly4053
100 μg
$183
View
BioLegend
407116
FC IgG2a
Mouse
Rat
IgG
RMG2a-62
100 μg
$194
View
BioLegend
406525
FC IgM
Mouse
Rat
IgG2a κ
RMM-1
25 μg
$89
View
BioLegend
348227
FC IgD
Human
Mouse
IgG2a κ
IA6-2
25 Tests
$159
View
Thermo Fisher Scientific
A21238
FC,IF IgM
Mouse
Goat
IgG
Polyclonal
250 μl
$139
View
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Alexa Fluor 647 Conjugation Kits


Product Type Company/SKU Applications Size / Price Details
Conjugation Kit Thermo Fisher Scientific
A20186
Labeling 5 x 100 μg
$484
View

Alexa Fluor 647 Conjugation Services


Product Type Company/SKU Applications Details
Conjugation Service Biotium
BIOTIUM-CONJ-AF647
N/A View
Conjugation Service Columbia Biosciences
COLUMBIA-CONJ-Alexa Fluor 647
Labeling View
Conjugation Service BioLegend
BL-CONJ-AF647
N/A View
Conjugation Service BD Biosciences
BD-CONJ-AF647
N/A View