ATTO 633
ATTO 633 (Ex-Max 629 nm/Em-Max 657 nm)
ATTO 633 from ATTO-TEC has an excitation peak at 629 nm and an emission peak at 657 nm. ATTO 633 belongs to a new generation of fluorescent labels for the red spectral region. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. Thus ATTO 633 is highly suitable for single-molecule detection applications and high-resolution microscopy such as SIM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. In common with most ATTO-labels, absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11, used in typical applications. ATTO 633 is a cationic dye. After coupling to a substrate the label carries a net electrical charge of +1. The label is moderately hydrophilic.
Excitation & emission spectra
Key facts
- Excitation maximum
- 629 nm
- Emission maximum
- 657 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Relative brightness
- 3 of 5
- Extinction coefficient
- 130000
- Quantum yield
- 0.64
- Fluorescence lifetime
- 3.3 ns
- Molecular weight
- 677
- Commercial antibodies
- 456 from 5 suppliers
ATTO 633 has an excitation maximum at 629 nm and an emission maximum at 657 nm, a Stokes shift of 28 nm. Its extinction coefficient is 130000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is ATTO 633?
- ATTO 633 from ATTO-TEC has an excitation peak at 629 nm and an emission peak at 657 nm. ATTO 633 belongs to a new generation of fluorescent labels for the red spectral region. Characteristic features of the label are strong absorption, high fluorescence quantum yield, and high thermal and photo-stability. Thus ATTO 633 is highly suitable for single-molecule detection applications and high-resolution microscopy such as SIM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more. In common with most ATTO-labels, absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11, used in typical applications. ATTO 633 is a cationic dye. After coupling to a substrate the label carries a net electrical charge of +1. The label is moderately hydrophilic.
- What laser excites ATTO 633?
- ATTO 633 is optimally excited by the 628-640 laser line. Its excitation maximum is 629 nm.
- What filter should I use to detect ATTO 633?
- A 660/20 filter captures the peak of the ATTO 633 emission near 657 nm with minimal spillover.
- How bright is ATTO 633?
- ATTO 633 has a relative brightness of 3 out of 5 (quantum yield 0.64).
- How many antibodies are available conjugated to ATTO 633?
- FluoroFinder tracks 456 commercially available ATTO 633 conjugates, searchable by target, clone, host and application.