ROS-ID Red
ROS-ID Red (Ex-Max 645 nm/Em-Max 662 nm)
ROS-ID Red Hypoxia is a fluorogenic, cell-permeant reagent used in kits from Enzo Life Sciences to detect hypoxic cells. The reagent becomes fluorescent under the activity of intracellular nitroreductase activity which converts the nitro group to hydroxylamine (NHOH) and an amino group (NH2) which releases the fluorescent probe. It has an excitation peak at 645 nm and an emission peak at 662 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 645 nm
- Emission maximum
- 662 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Commercial antibodies
- 1 from 1 supplier
ROS-ID Red has an excitation maximum at 645 nm and an emission maximum at 662 nm, a Stokes shift of 17 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is ROS-ID Red?
- ROS-ID Red Hypoxia is a fluorogenic, cell-permeant reagent used in kits from Enzo Life Sciences to detect hypoxic cells. The reagent becomes fluorescent under the activity of intracellular nitroreductase activity which converts the nitro group to hydroxylamine (NHOH) and an amino group (NH2) which releases the fluorescent probe. It has an excitation peak at 645 nm and an emission peak at 662 nm.
- What laser excites ROS-ID Red?
- ROS-ID Red is optimally excited by the 628-640 laser line. Its excitation maximum is 645 nm.
- What filter should I use to detect ROS-ID Red?
- A 660/20 filter captures the peak of the ROS-ID Red emission near 662 nm with minimal spillover.
- How many antibodies are available conjugated to ROS-ID Red?
- FluoroFinder tracks 1 commercially available ROS-ID Red conjugates, searchable by target, clone, host and application.