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SureLight PE Cyanine 5.0

SureLight PE-665 (Ex-Max 565 nm/Em-Max 665 nm)

SureLight PE-665 (PE-Cyanine 5) from Columbia Biosciences is a tandem with an excitation peak at 488 and 565 nm and an emission peak at 665 nm. SureLight PE-665 is available in antibody conjugation kits for use in flow cytometry.

Excitation & emission spectra

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Key facts

Excitation maximum
565 nm
Emission maximum
665 nm
Optimal laser line
488-561
Optimal filter
660/20
Relative brightness
5 of 5
Molecular weight
250000
Commercial conjugates
5 from 1 supplier

SureLight PE Cyanine 5.0 has an excitation maximum at 565 nm and an emission maximum at 665 nm, a Stokes shift of 100 nm. It is optimally excited by the 488-561 laser line.

Frequently asked questions

What is SureLight PE Cyanine 5.0?
SureLight PE-665 (PE-Cyanine 5) from Columbia Biosciences is a tandem with an excitation peak at 488 and 565 nm and an emission peak at 665 nm. SureLight PE-665 is available in antibody conjugation kits for use in flow cytometry.
What laser excites SureLight PE Cyanine 5.0?
SureLight PE Cyanine 5.0 is optimally excited by the 488-561 laser line. Its excitation maximum is 565 nm.
What filter should I use to detect SureLight PE Cyanine 5.0?
A 660/20 filter captures the peak of the SureLight PE Cyanine 5.0 emission near 665 nm with minimal spillover.
How bright is SureLight PE Cyanine 5.0?
SureLight PE Cyanine 5.0 has a relative brightness of 5 out of 5.
How many antibodies are available conjugated to SureLight PE Cyanine 5.0?
FluoroFinder tracks 5 commercially available SureLight PE Cyanine 5.0 conjugates, searchable by target, clone, host and application.