640

Laser Line

660/20

Common Filter

649

Excitation Max

667

Emission Max

7,669

Antibodies

9

Conjugation Kits

1

Services


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About Cy5


Cyanine 5 (Cy®5) is a red-emitting fluorophore that can be excited by the 633 or 640 nm red laser and captured with a 660/20 nm bandpass filter. Cy5 has an excitation peak at 649 nm and an emission peak at 667 nm, and is spectrally similar to APC and Alexa Fluor 647. Cy5 is bright and can be a used for a variety of applications. A very common use for Cy5 is as the acceptor molecule in the PE-Cy5 tandem fluorophore where the broad excitation of PE paired with the bright emission of Cy5 and the long stoke’s shift make PE-Cy5 an asset in flow cytometry. While Cy5 can be used for flow cytometry, APC has been a mainstay in the application and leaves little room for other dyes with similar emission properties. Despite this, it does often get used in the form of PE-Cy5, which is a very popular fluorophore for flow cytometry. Other applications of Cy5 alone include imaging, microscopy, and genomic applications where the dye is conjugated to a protein or nucleic acid probe. Cyanine dyes are known to be photostable for microscopy or imaging, as well as being resistant to harsh dehydration and embedding conditions that come with mounting tissue sections. Additionally, Cy5 comes in a wide variety of modified formats that allow for easy conjugations. The Cyanine dye family are registered trademarks of GE Healthcare.


Popular Cy5 Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
SouthernBiotech
9536-15
FC,ICC,IHC-P,IHC-Fr CD8
Human
Mouse
IgG1
RFT-8
100 Tests
$260
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SouthernBiotech
9522-15
FC,ICC,IHC-Fr CD4
Human
Mouse
IgG1
RFT4
100 Tests
$260
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Abcam
ab24845
FC,IHC-Fr CD19
Human
Mouse
IgG1
SJ25C1
50 Tests
$372
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Abcam
ab24847
Activation,FC,IHC-Fr CD3
Human
Mouse
IgG1
UCHT1
50 Tests
$378
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Abcam
ab25532
FC,IHC-Fr,Functional CD4
Mouse
Rat
IgG2b
GK1.5
50 μg
$186
View
Abcam
ab24863
FC,IHC-Fr CD8
Human
Mouse
IgG1
RFT-8
50 Tests
$185
View
Bioss
bs-4834R-Cy5
FC,IF,IHC CD73
Human,Mouse,Rat,Canine,Chicken
Rabbit
IgG
100 μl
$470
View
Bioss
bs-5758R-Cy5
IHC-P Fibroblast Activation Protein alpha
Human,Mouse,Rat
Rabbit
IgG
100 μl
$470
View
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