Thiazole Red Homodimer
Thiazole Red Homodimer (Ex-Max 642 nm/Em-Max 661 nm)
Thiazole Red Homodimer (TOTO-3) is a bis-intercalating cell-impermeant nucleic acid stain used for counterstaining fixed cell nuclei or assessing viability. It has an excitation peak at 642 nm and an emission peak at 657 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 642 nm
- Emission maximum
- 661 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
Thiazole Red Homodimer has an excitation maximum at 642 nm and an emission maximum at 661 nm, a Stokes shift of 19 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Thiazole Red Homodimer?
- Thiazole Red Homodimer (TOTO-3) is a bis-intercalating cell-impermeant nucleic acid stain used for counterstaining fixed cell nuclei or assessing viability. It has an excitation peak at 642 nm and an emission peak at 657 nm.
- What laser excites Thiazole Red Homodimer?
- Thiazole Red Homodimer is optimally excited by the 628-640 laser line. Its excitation maximum is 642 nm.
- What filter should I use to detect Thiazole Red Homodimer?
- A 660/20 filter captures the peak of the Thiazole Red Homodimer emission near 661 nm with minimal spillover.