640

Laser Line

660/20

Common Filter

641

Excitation Max

660

Emission Max


Spectra Viewer

About Calcein Deep Red AM


Calcein Deep Red is a red fluorescent molecule that penetrates the membranes of dead cells and is used to evaluate cell membrane integrity as well as esterase activity. Closely related is Calcein Deep Red AM, which due to the acetoxymethyl (AM) modification, can passively diffuse across the membranes of live cells. While inside the live cell, Calcein Deep Red AM is hydrolyzed by esterase into the fluorescent Calcein Deep Red which can then be measured via fluorescent microscopy, flow cytometer, or fluorescent microplate reader.