Calcein Deep Red AM
Calcein Deep Red AM (Ex-Max 641 nm/Em-Max 660 nm)
Calcein Deep Red, AM, is a fluorogenic deep red emitting fluorescent molecule (ex 641/ em 660) that penetrates the membranes of live cells. Once inside the live cell, Calcein Deep Red, AM is hydrolyzed by intracellular esterases into the fluorescent Calcein Deep Red which can then be measured via Fluorescence Microscopy, flow cytometer, or fluorescent microplate reader. Reagents that contain an acetoxymethyl ester (AM) or diacetate (DA) side chain, are useful indicators of cell health and vitality, especially when used together with a probe for cell membrane integrity like an impermeant nucleic acid stain or fixable live/dead probe.
Excitation & emission spectra
Key facts
- Excitation maximum
- 641 nm
- Emission maximum
- 660 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Molecular weight
- 373.66
Calcein Deep Red AM has an excitation maximum at 641 nm and an emission maximum at 660 nm, a Stokes shift of 19 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Calcein Deep Red AM?
- Calcein Deep Red, AM, is a fluorogenic deep red emitting fluorescent molecule (ex 641/ em 660) that penetrates the membranes of live cells. Once inside the live cell, Calcein Deep Red, AM is hydrolyzed by intracellular esterases into the fluorescent Calcein Deep Red which can then be measured via Fluorescence Microscopy, flow cytometer, or fluorescent microplate reader. Reagents that contain an acetoxymethyl ester (AM) or diacetate (DA) side chain, are useful indicators of cell health and vitality, especially when used together with a probe for cell membrane integrity like an impermeant nucleic acid stain or fixable live/dead probe.
- What laser excites Calcein Deep Red AM?
- Calcein Deep Red AM is optimally excited by the 628-640 laser line. Its excitation maximum is 641 nm.
- What filter should I use to detect Calcein Deep Red AM?
- A 660/20 filter captures the peak of the Calcein Deep Red AM emission near 660 nm with minimal spillover.