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NovaFluor Yellow 660

NovaFluor Yellow 660 (Ex-Max 552 nm/Em-Max 663 nm)

The NovaFluor family of fluorescent polymers from Thermo Fisher Scientific are a novel reagent for traditional and spectral flow cytometry. These fluorophores are comprised of a DNA backbone polymer integrated with fluorophore substituted nucleosides to create a potent donor molecule. Tandem fluophores with long Stokes' Shift enable brighter, spectrally discrete, tunable fluorescent polymers as an alternative to traditional simple organic structures, protein-based fluorophores as well as the Horizon Brilliant™ family of fluorescent polymers from BD. NovaFluor Yellow 660 is designed to be discreetly excited by the 561nm laser and has an excitation peak at 552 nm and an emission peak at 663 nm.

Excitation & emission spectra

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Key facts

Excitation maximum
552 nm
Emission maximum
663 nm
Optimal laser line
561
Optimal filter
660/20
Relative brightness
2 of 5
Commercial conjugates
137 from 1 supplier

NovaFluor Yellow 660 has an excitation maximum at 552 nm and an emission maximum at 663 nm, a Stokes shift of 111 nm. It is optimally excited by the 561 laser line.

Frequently asked questions

What is NovaFluor Yellow 660?
The NovaFluor family of fluorescent polymers from Thermo Fisher Scientific are a novel reagent for traditional and spectral flow cytometry. These fluorophores are comprised of a DNA backbone polymer integrated with fluorophore substituted nucleosides to create a potent donor molecule. Tandem fluophores with long Stokes' Shift enable brighter, spectrally discrete, tunable fluorescent polymers as an alternative to traditional simple organic structures, protein-based fluorophores as well as the Horizon Brilliant™ family of fluorescent polymers from BD. NovaFluor Yellow 660 is designed to be discreetly excited by the 561nm laser and has an excitation peak at 552 nm and an emission peak at 663 nm.
What laser excites NovaFluor Yellow 660?
NovaFluor Yellow 660 is optimally excited by the 561 laser line. Its excitation maximum is 552 nm.
What filter should I use to detect NovaFluor Yellow 660?
A 660/20 filter captures the peak of the NovaFluor Yellow 660 emission near 663 nm with minimal spillover.
How bright is NovaFluor Yellow 660?
NovaFluor Yellow 660 has a relative brightness of 2 out of 5.
How many antibodies are available conjugated to NovaFluor Yellow 660?
FluoroFinder tracks 137 commercially available NovaFluor Yellow 660 conjugates, searchable by target, clone, host and application.