CypHer5E
CypHer5E (Ex-Max 647 nm/Em-Max 667 nm)
CypHer5E is a pH-sensitive cyanine dye derivative used for reporting cell surface internalization into acidic endosomes. It has an excitation peak at 647 nm and emission peak at 667 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 647 nm
- Emission maximum
- 667 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
CypHer5E has an excitation maximum at 647 nm and an emission maximum at 667 nm, a Stokes shift of 20 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is CypHer5E?
- CypHer5E is a pH-sensitive cyanine dye derivative used for reporting cell surface internalization into acidic endosomes. It has an excitation peak at 647 nm and emission peak at 667 nm.
- What laser excites CypHer5E?
- CypHer5E is optimally excited by the 628-640 laser line. Its excitation maximum is 647 nm.
- What filter should I use to detect CypHer5E?
- A 660/20 filter captures the peak of the CypHer5E emission near 667 nm with minimal spillover.