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LysoView 640

LysoView™ 640 (Ex-Max 640 nm/Em-Max 671 nm)

Biotium's LysoView™ dyes are cell-permeant alkaline probes that localize to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoView™ 640 has an excitation peak at 640 nm and an emission peak at 671 nm.

Excitation & emission spectra

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Key facts

Excitation maximum
640 nm
Emission maximum
671 nm
Optimal laser line
628-640
Optimal filter
660/20
Commercial antibodies
2 from 1 supplier

LysoView 640 has an excitation maximum at 640 nm and an emission maximum at 671 nm, a Stokes shift of 31 nm. It is optimally excited by the 628-640 laser line.

Frequently asked questions

What is LysoView 640?
Biotium's LysoView™ dyes are cell-permeant alkaline probes that localize to acidic compartments inside the cell, such as endosomes and lysosomes. These types of probes are designed for live-cell imaging and do not typically survive fixation and permeabilization. LysoView™ 640 has an excitation peak at 640 nm and an emission peak at 671 nm.
What laser excites LysoView 640?
LysoView 640 is optimally excited by the 628-640 laser line. Its excitation maximum is 640 nm.
What filter should I use to detect LysoView 640?
A 660/20 filter captures the peak of the LysoView 640 emission near 671 nm with minimal spillover.
How many antibodies are available conjugated to LysoView 640?
FluoroFinder tracks 2 commercially available LysoView 640 conjugates, searchable by target, clone, host and application.