About NucSpot Far-Red

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Biotium’s NucSpot Far-Red is a cell membrane-impermeant dye that enters cells with compromised membranes and is used for live/dead discrimination or DNA content analysis. This dye was developed as an alternative to 7-AAD with more advantageous spectral properties for reduced spillover. Since this dye stains cells with compromised membranes, it can be used with fixed cells, permeabilized cells, or as a stain for dead cell detection with flow cytometry, microscopy, or imaging applications. The more general NucSpot family of dyes has a few more members that have different functions compared to NucSpot Far-Red. NucSpot 470 is similar in function but emits in the green area of the visible light spectrum, while NucSpot Live dyes are cell membrane-permeant DNA stains used for live cell staining. Check out the other members of this dye line in FluoroFinder’s Dye Directory or on our Spectra Viewer.

Dyes similar to NucSpot Far-Red based on emission

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Alexa Fluor 647 APC ATTO 647 ATTO 647N BactoView Live Red Brilliant Blue 660-P2 BDY 650-X SE BODIPY 650/665 Brilliant Ultraviolet 661 Calcein Deep Red AM CellBrite Fix 640 CellBrite Red CellBrite Steady 650 CellROX Deep Red CellTrace Far Red CellVue Maroon CellVue Plum CF640R CF647 CF647ST CF650 Chromeo 642 CoraLite Plus 647 CPN680 Red Cy5 Cyanine 5 SE Cyanine 647 CypHer5E DiD DY-520XL DY-521XL DyLight 633 DyLight 649 DyLight 650 Dyomics 647 eFluor 660 eFluor 670 Cell Proliferation ER-ID Red ExoBrite 640/660 EV Membrane Stain Flash Phalloidin NIR 647 FluoProbes647H Fura Red AM (Ca2+free) FVS660 FxCycle Far Red HiLyte 647 iFluor 647 IRDye 650 iRFP 670 Janelia Fluor 646 LipidSpot 610 Live/Dead Fix Far Red LysoView 633 LysoView 640 MaxLight 650 MemBrite Fix 640/660 MemBrite Fix ST 650/665 MitoView 650 NeuroVue Maroon Nile Blue NirFP NL 637 NovaFluor Blue 660 / 120S NovaFluor Blue 660 / 40S NovaFluor Red 660 NovaFluor Yellow 660 NucSpot 640 Oyster 650 SureLight P3 PE-Alexa 647 PE-Cy5 PE-Dyomics 647 PerCP PhenoVue 641 Mitochondrial Stain PromoFluor 647P ReadiLink 647-674 ROS-ID Red RPE-CF647T Seta-650-NHS Seta-PerCP-680 Seta-R-PE-670 StarBright UltraViolet 665 SureLight APC SureLight PE Cyanine 5.0 Thiazole Red Homodimer TO-Pro 3 TOTO-3 Tri-Color Vio667 VioBright 667 VivaFix 649/660