NucSpot Far-Red
NucSpot Far-Red (Ex-Max 596 nm/Em-Max 667 nm)
Biotium's NucSpot Far-Red is a cell membrane-impermeant dye that enters cells with compromised membranes and is used for live/dead discrimination or DNA content analysis. This dye was developed as an alternative to 7-AAD with more advantageous spectral properties for reduced spillover. Since this dye stains cells with compromised membranes, it can be used with fixed cells, permeabilized cells, or as a stain for dead cell detection with flow cytometry and fluorescence microscopy applications. It has an excitation peak at 596 nm and an emission peak at 667 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 596 nm
- Emission maximum
- 667 nm
- Optimal laser line
- 561-594
- Optimal filter
- 660/20
NucSpot Far-Red has an excitation maximum at 596 nm and an emission maximum at 667 nm, a Stokes shift of 71 nm. It is optimally excited by the 561-594 laser line.
Frequently asked questions
- What is NucSpot Far-Red?
- Biotium's NucSpot Far-Red is a cell membrane-impermeant dye that enters cells with compromised membranes and is used for live/dead discrimination or DNA content analysis. This dye was developed as an alternative to 7-AAD with more advantageous spectral properties for reduced spillover. Since this dye stains cells with compromised membranes, it can be used with fixed cells, permeabilized cells, or as a stain for dead cell detection with flow cytometry and fluorescence microscopy applications. It has an excitation peak at 596 nm and an emission peak at 667 nm.
- What laser excites NucSpot Far-Red?
- NucSpot Far-Red is optimally excited by the 561-594 laser line. Its excitation maximum is 596 nm.
- What filter should I use to detect NucSpot Far-Red?
- A 660/20 filter captures the peak of the NucSpot Far-Red emission near 667 nm with minimal spillover.