NucSpot Far-Red Dye Profile


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About NucSpot Far-Red

Biotium’s NucSpot Far-Red is a cell membrane-impermeant dye that enters cells with compromised membranes and is used for live/dead discrimination or DNA content analysis. This dye was developed as an alternative to 7-AAD with more advantageous spectral properties for reduced spillover. Since this dye stains cells with compromised membranes, it can be used with fixed cells, permeabilized cells, or as a stain for dead cell detection with flow cytometry, microscopy, or imaging applications. The more general NucSpot family of dyes has a few more members that have different functions compared to NucSpot Far-Red. NucSpot 470 is similar in function but emits in the green area of the visible light spectrum, while NucSpot Live dyes are cell membrane-permeant DNA stains used for live cell staining. Check out the other members of this dye line in FluoroFinder’s Dye Directory or on our Spectra Viewer.