Cyanine 5 SE
Cyanine 5 SE (Ex-Max 643 nm/Em-Max 663 nm)
Cyanine 5 has an excitation peak at 649 nm and an emission peak at 667 nm. Spectrally its similar to Alexa Fluor™ 647.
Excitation & emission spectra
Key facts
- Excitation maximum
- 643 nm
- Emission maximum
- 663 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Relative brightness
- 3 of 5
- Extinction coefficient
- 250000
- Quantum yield
- 0.2
Cyanine 5 SE has an excitation maximum at 643 nm and an emission maximum at 663 nm, a Stokes shift of 20 nm. Its extinction coefficient is 250000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Cyanine 5 SE?
- Cyanine 5 has an excitation peak at 649 nm and an emission peak at 667 nm. Spectrally its similar to Alexa Fluor™ 647.
- What laser excites Cyanine 5 SE?
- Cyanine 5 SE is optimally excited by the 628-640 laser line. Its excitation maximum is 643 nm.
- What filter should I use to detect Cyanine 5 SE?
- A 660/20 filter captures the peak of the Cyanine 5 SE emission near 663 nm with minimal spillover.
- How bright is Cyanine 5 SE?
- Cyanine 5 SE has a relative brightness of 3 out of 5 (quantum yield 0.2).