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CF650

CF®650 (Ex-Max 650 nm/Em-Max 670 nm)

CF®650 from Biotium is a fluorophore with an excitation peak at 650 nm and an emission peak at 670 nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Alexa Fluor™ 647, DyLight™ 649 and Cy5.

Excitation & emission spectra

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Key facts

Excitation maximum
650 nm
Emission maximum
670 nm
Optimal laser line
628-640
Optimal filter
660/20
Relative brightness
4 of 5
Commercial conjugates
1 from 1 supplier

CF650 has an excitation maximum at 650 nm and an emission maximum at 670 nm, a Stokes shift of 20 nm. It is optimally excited by the 628-640 laser line.

Frequently asked questions

What is CF650?
CF®650 from Biotium is a fluorophore with an excitation peak at 650 nm and an emission peak at 670 nm. It can be useful in both fluorescence microscopy and flow cytometry. It is spectrally similar to Alexa Fluor™ 647, DyLight™ 649 and Cy5.
What laser excites CF650?
CF650 is optimally excited by the 628-640 laser line. Its excitation maximum is 650 nm.
What filter should I use to detect CF650?
A 660/20 filter captures the peak of the CF650 emission near 670 nm with minimal spillover.
How bright is CF650?
CF650 has a relative brightness of 4 out of 5.
How many antibodies are available conjugated to CF650?
FluoroFinder tracks 1 commercially available CF650 conjugates, searchable by target, clone, host and application.