Thiazole Red
Thiazole Red (Ex-Max 642 nm/Em-Max 657 nm)
Thiazole Red (TO-PRO-3) is an intercalating cell-impermeant nucleic acid stain used for viablity or apoptosis detection. It has an excitation peak at 642 nm and an emission peak at 657 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 642 nm
- Emission maximum
- 657 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
Thiazole Red has an excitation maximum at 642 nm and an emission maximum at 657 nm, a Stokes shift of 15 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Thiazole Red?
- Thiazole Red (TO-PRO-3) is an intercalating cell-impermeant nucleic acid stain used for viablity or apoptosis detection. It has an excitation peak at 642 nm and an emission peak at 657 nm.
- What laser excites Thiazole Red?
- Thiazole Red is optimally excited by the 628-640 laser line. Its excitation maximum is 642 nm.
- What filter should I use to detect Thiazole Red?
- A 660/20 filter captures the peak of the Thiazole Red emission near 657 nm with minimal spillover.