TagRFP657
TagRFP657 (Ex-Max 607 nm/Em-Max 653 nm)
TagRFP657 is a monomeric fluorescent protein derived from the sea anemonies, Entacmaea quadricolor. It has an excitation peak at 607 nm and an emission peak at 653 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 607 nm
- Emission maximum
- 653 nm
- Optimal laser line
- 561-594
- Optimal filter
- 660/20
- Extinction coefficient
- 34000
- Quantum yield
- 0.1
- Molecular weight
- 26300
TagRFP657 has an excitation maximum at 607 nm and an emission maximum at 653 nm, a Stokes shift of 46 nm. Its extinction coefficient is 34000 M⁻¹cm⁻¹. It is optimally excited by the 561-594 laser line.
Frequently asked questions
- What is TagRFP657?
- TagRFP657 is a monomeric fluorescent protein derived from the sea anemonies, Entacmaea quadricolor. It has an excitation peak at 607 nm and an emission peak at 653 nm.
- What laser excites TagRFP657?
- TagRFP657 is optimally excited by the 561-594 laser line. Its excitation maximum is 607 nm.
- What filter should I use to detect TagRFP657?
- A 660/20 filter captures the peak of the TagRFP657 emission near 653 nm with minimal spillover.