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TagRFP657

TagRFP657 (Ex-Max 607 nm/Em-Max 653 nm)

TagRFP657 is a monomeric fluorescent protein derived from the sea anemonies, Entacmaea quadricolor. It has an excitation peak at 607 nm and an emission peak at 653 nm.

Excitation & emission spectra

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Key facts

Excitation maximum
607 nm
Emission maximum
653 nm
Optimal laser line
561-594
Optimal filter
660/20
Extinction coefficient
34000
Quantum yield
0.1
Molecular weight
26300

TagRFP657 has an excitation maximum at 607 nm and an emission maximum at 653 nm, a Stokes shift of 46 nm. Its extinction coefficient is 34000 M⁻¹cm⁻¹. It is optimally excited by the 561-594 laser line.

Frequently asked questions

What is TagRFP657?
TagRFP657 is a monomeric fluorescent protein derived from the sea anemonies, Entacmaea quadricolor. It has an excitation peak at 607 nm and an emission peak at 653 nm.
What laser excites TagRFP657?
TagRFP657 is optimally excited by the 561-594 laser line. Its excitation maximum is 607 nm.
What filter should I use to detect TagRFP657?
A 660/20 filter captures the peak of the TagRFP657 emission near 653 nm with minimal spillover.