BODIPY 650/665
BODIPY 650/665 (Ex-Max 644 nm/Em-Max 661 nm)
BODIPY 650/665 is used as a lipophilic probe for polar and neutral lipids typically used to detect oil and nonpolar lipid droplets in live or fixed cells. It has an excitation peak at 650 nm and an emission peak at 655 nm. The succinimidyl ester form of this dye can be used to conjugate hydrophobic proteins or lipids.
Excitation & emission spectra
Key facts
- Excitation maximum
- 623 nm
- Emission maximum
- 661 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Molecular weight
- 643.45
BODIPY 650/665 has an excitation maximum at 623 nm and an emission maximum at 661 nm, a Stokes shift of 38 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is BODIPY 650/665?
- BODIPY 650/665 is used as a lipophilic probe for polar and neutral lipids typically used to detect oil and nonpolar lipid droplets in live or fixed cells. It has an excitation peak at 650 nm and an emission peak at 655 nm. The succinimidyl ester form of this dye can be used to conjugate hydrophobic proteins or lipids.
- What laser excites BODIPY 650/665?
- BODIPY 650/665 is optimally excited by the 628-640 laser line. Its excitation maximum is 623 nm.
- What filter should I use to detect BODIPY 650/665?
- A 660/20 filter captures the peak of the BODIPY 650/665 emission near 661 nm with minimal spillover.