Sytox Red
Sytox Red (Ex-Max 639 nm/Em-Max 655 nm)
Sytox Red from Thermo Fisher Scientific is a cell-impermeant, fluorogenic nucleic acid stain that can be used as a viability dye for use in flow cytometry and as a nuclear counterstain in fluorescence microscopy. It has an excitation peak at 639 nm an emission peak at 655 nm, similar to To-Pro-3.
Excitation & emission spectra
Key facts
- Excitation maximum
- 639 nm
- Emission maximum
- 655 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
Sytox Red has an excitation maximum at 639 nm and an emission maximum at 655 nm, a Stokes shift of 16 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Sytox Red?
- Sytox Red from Thermo Fisher Scientific is a cell-impermeant, fluorogenic nucleic acid stain that can be used as a viability dye for use in flow cytometry and as a nuclear counterstain in fluorescence microscopy. It has an excitation peak at 639 nm an emission peak at 655 nm, similar to To-Pro-3.
- What laser excites Sytox Red?
- Sytox Red is optimally excited by the 628-640 laser line. Its excitation maximum is 639 nm.
- What filter should I use to detect Sytox Red?
- A 660/20 filter captures the peak of the Sytox Red emission near 655 nm with minimal spillover.