TOTO-3
TOTO-3 (Ex-Max 642 nm/Em-Max 660 nm)
TOTO-3 from Thermo Fisher Scientific is an intercalating, fluorogenic DNA dye used for counterstaining fixed cell nucleii or for assessing viability in flow cytometry. It has an excitation peak at 642 nm and an emission peak at 660 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 642 nm
- Emission maximum
- 660 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Commercial antibodies
- 1 from 1 supplier
TOTO-3 has an excitation maximum at 642 nm and an emission maximum at 660 nm, a Stokes shift of 18 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is TOTO-3?
- TOTO-3 from Thermo Fisher Scientific is an intercalating, fluorogenic DNA dye used for counterstaining fixed cell nucleii or for assessing viability in flow cytometry. It has an excitation peak at 642 nm and an emission peak at 660 nm.
- What laser excites TOTO-3?
- TOTO-3 is optimally excited by the 628-640 laser line. Its excitation maximum is 642 nm.
- What filter should I use to detect TOTO-3?
- A 660/20 filter captures the peak of the TOTO-3 emission near 660 nm with minimal spillover.
- How many antibodies are available conjugated to TOTO-3?
- FluoroFinder tracks 1 commercially available TOTO-3 conjugates, searchable by target, clone, host and application.