Cyanine 647
Cyanine 647 (Ex-Max 652 nm/Em-Max 668 nm)
Cyanine 647 has an excitation peak at 649 nm and an emission peak at 667 nm. Spectrally its similar to Alexa Fluor™ 647 and Cy5.
Excitation & emission spectra
Key facts
- Excitation maximum
- 652 nm
- Emission maximum
- 668 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Relative brightness
- 4 of 5
- Commercial conjugates
- 4 from 1 supplier
Cyanine 647 has an excitation maximum at 652 nm and an emission maximum at 668 nm, a Stokes shift of 16 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Cyanine 647?
- Cyanine 647 has an excitation peak at 649 nm and an emission peak at 667 nm. Spectrally its similar to Alexa Fluor™ 647 and Cy5.
- What laser excites Cyanine 647?
- Cyanine 647 is optimally excited by the 628-640 laser line. Its excitation maximum is 652 nm.
- What filter should I use to detect Cyanine 647?
- A 660/20 filter captures the peak of the Cyanine 647 emission near 668 nm with minimal spillover.
- How bright is Cyanine 647?
- Cyanine 647 has a relative brightness of 4 out of 5.
- How many antibodies are available conjugated to Cyanine 647?
- FluoroFinder tracks 4 commercially available Cyanine 647 conjugates, searchable by target, clone, host and application.