CellBrite Fix 640
CellBrite Fix 640 (Ex-Max 638 nm/Em-Max 667 nm)
Biotium's CellBrite Fix dyes are versatile amine-reactive dyes that are primarily used for live cell outer plasma membrane staining but can withstand fixation and detergent permeabilization. The unique benefit here is that since CellBrite Fix dyes can withstand permeabilization, extracellular staining can be combined with intracellular staining techniques. This dye line shares similarities with Biotium's MemBrite Fix and MemBrite Fix-ST line, but there are a few key differences. CellBrite Fix stains are easy to use and require a single staining step while MemBrite Fix dyes require a two-step staining protocol. While ease of use is important, MemBrite Fix and Membrite Fix-ST dyes have more colors to choose from and they do not associate with lipids in the membrane which leads to lower cytoplasmic background after permeabilization.
Excitation & emission spectra
Key facts
- Excitation maximum
- 638 nm
- Emission maximum
- 667 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Commercial antibodies
- 2 from 1 supplier
CellBrite Fix 640 has an excitation maximum at 638 nm and an emission maximum at 667 nm, a Stokes shift of 29 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is CellBrite Fix 640?
- Biotium's CellBrite Fix dyes are versatile amine-reactive dyes that are primarily used for live cell outer plasma membrane staining but can withstand fixation and detergent permeabilization. The unique benefit here is that since CellBrite Fix dyes can withstand permeabilization, extracellular staining can be combined with intracellular staining techniques. This dye line shares similarities with Biotium's MemBrite Fix and MemBrite Fix-ST line, but there are a few key differences. CellBrite Fix stains are easy to use and require a single staining step while MemBrite Fix dyes require a two-step staining protocol. While ease of use is important, MemBrite Fix and Membrite Fix-ST dyes have more colors to choose from and they do not associate with lipids in the membrane which leads to lower cytoplasmic background after permeabilization.
- What laser excites CellBrite Fix 640?
- CellBrite Fix 640 is optimally excited by the 628-640 laser line. Its excitation maximum is 638 nm.
- What filter should I use to detect CellBrite Fix 640?
- A 660/20 filter captures the peak of the CellBrite Fix 640 emission near 667 nm with minimal spillover.
- How many antibodies are available conjugated to CellBrite Fix 640?
- FluoroFinder tracks 2 commercially available CellBrite Fix 640 conjugates, searchable by target, clone, host and application.