SureLight P3
P3 (Ex-Max 614 nm/Em-Max 662 nm)
SureLight P3 from Columbia Biosciences is a unique structure combining C-PC and APC fluorescent proteins, with an excitation peak at 614 nm and an emission peak at 662 nm. SureLight P3 is available in antibody conjugation kits for use in flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 614 nm
- Emission maximum
- 662 nm
- Optimal laser line
- 594
- Optimal filter
- 660/20
- Relative brightness
- 5 of 5
- Extinction coefficient
- 96400000
- Commercial antibodies
- 8 from 2 suppliers
SureLight P3 has an excitation maximum at 614 nm and an emission maximum at 662 nm, a Stokes shift of 48 nm. Its extinction coefficient is 96400000 M⁻¹cm⁻¹. It is optimally excited by the 594 laser line.
Frequently asked questions
- What is SureLight P3?
- SureLight P3 from Columbia Biosciences is a unique structure combining C-PC and APC fluorescent proteins, with an excitation peak at 614 nm and an emission peak at 662 nm. SureLight P3 is available in antibody conjugation kits for use in flow cytometry.
- What laser excites SureLight P3?
- SureLight P3 is optimally excited by the 594 laser line. Its excitation maximum is 614 nm.
- What filter should I use to detect SureLight P3?
- A 660/20 filter captures the peak of the SureLight P3 emission near 662 nm with minimal spillover.
- How bright is SureLight P3?
- SureLight P3 has a relative brightness of 5 out of 5.
- How many antibodies are available conjugated to SureLight P3?
- FluoroFinder tracks 8 commercially available SureLight P3 conjugates, searchable by target, clone, host and application.