DyLight 650
DyLight™ 650 (Ex-Max 652 nm/Em-Max 672 nm)
DyLight™ 650 is a red-emitting fluorophore that excited by the 640 nm laser and collected using a 670/30 nm bandpass filter. DyLight™ 650 has an excitation peak at 652 nm and an emission peak at 672 nm, and is spectrally similar to Alexa Fluor™ 647 and Cy5. DyLight™ 650 is most commonly used in flow cytometery and fluorescence microscopy applications.
Excitation & emission spectra
Key facts
- Excitation maximum
- 652 nm
- Emission maximum
- 672 nm
- Optimal laser line
- 628-640
- Optimal filter
- 695/40
- Relative brightness
- 4 of 5
- Extinction coefficient
- 250000
- Commercial conjugates
- 1.4k from 13 suppliers
DyLight 650 has an excitation maximum at 652 nm and an emission maximum at 672 nm, a Stokes shift of 20 nm. Its extinction coefficient is 250000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is DyLight 650?
- DyLight™ 650 is a red-emitting fluorophore that excited by the 640 nm laser and collected using a 670/30 nm bandpass filter. DyLight™ 650 has an excitation peak at 652 nm and an emission peak at 672 nm, and is spectrally similar to Alexa Fluor™ 647 and Cy5. DyLight™ 650 is most commonly used in flow cytometery and fluorescence microscopy applications.
- What laser excites DyLight 650?
- DyLight 650 is optimally excited by the 628-640 laser line. Its excitation maximum is 652 nm.
- What filter should I use to detect DyLight 650?
- A 695/40 filter captures the peak of the DyLight 650 emission near 672 nm with minimal spillover.
- How bright is DyLight 650?
- DyLight 650 has a relative brightness of 4 out of 5.
- How many antibodies are available conjugated to DyLight 650?
- FluoroFinder tracks 1414 commercially available DyLight 650 conjugates, searchable by target, clone, host and application.