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PromoFluor 647P

PromoFluor 647P (Ex-Max 653 nm/Em-Max 673 nm)

PromoFluor 647P is a fluorophore with an excitation peak at 654 nm and an emission peak at 672 nm. It is equivalent to Alexa Fluor™ 647 and Cy5 in size, brightness and application. It can be used with the following analysis platforms: Flow Cytometry and Fluorescence Microscopy.

Excitation & emission spectra

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Key facts

Excitation maximum
653 nm
Emission maximum
673 nm
Optimal laser line
628-640
Optimal filter
660/20
Relative brightness
4 of 5
Quantum yield
0.399
Commercial antibodies
5 from 2 suppliers

PromoFluor 647P has an excitation maximum at 653 nm and an emission maximum at 673 nm, a Stokes shift of 20 nm. It is optimally excited by the 628-640 laser line.

Frequently asked questions

What is PromoFluor 647P?
PromoFluor 647P is a fluorophore with an excitation peak at 654 nm and an emission peak at 672 nm. It is equivalent to Alexa Fluor™ 647 and Cy5 in size, brightness and application. It can be used with the following analysis platforms: Flow Cytometry and Fluorescence Microscopy.
What laser excites PromoFluor 647P?
PromoFluor 647P is optimally excited by the 628-640 laser line. Its excitation maximum is 653 nm.
What filter should I use to detect PromoFluor 647P?
A 660/20 filter captures the peak of the PromoFluor 647P emission near 673 nm with minimal spillover.
How bright is PromoFluor 647P?
PromoFluor 647P has a relative brightness of 4 out of 5 (quantum yield 0.399).
How many antibodies are available conjugated to PromoFluor 647P?
FluoroFinder tracks 5 commercially available PromoFluor 647P conjugates, searchable by target, clone, host and application.