dsRed
dsRed (Ex-Max 558 nm/Em-Max 583 nm)
DsRed (Red Fluorescent Protein, RFP) is a tetrameric fluorescent protein first isolated from sea anemone of the Discosoma genus. It has an excitation peak at 558 nm and an emisison peak at 583 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 558 nm
- Emission maximum
- 583 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
- Extinction coefficient
- 72500
- Quantum yield
- 0.68
- Molecular weight
- 29500
dsRed has an excitation maximum at 558 nm and an emission maximum at 583 nm, a Stokes shift of 25 nm. Its extinction coefficient is 72500 M⁻¹cm⁻¹. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is dsRed?
- DsRed (Red Fluorescent Protein, RFP) is a tetrameric fluorescent protein first isolated from sea anemone of the Discosoma genus. It has an excitation peak at 558 nm and an emisison peak at 583 nm.
- What laser excites dsRed?
- dsRed is optimally excited by the 561 laser line. Its excitation maximum is 558 nm.
- What filter should I use to detect dsRed?
- A 585/42 filter captures the peak of the dsRed emission near 583 nm with minimal spillover.