Alizarin Red
Alizarin Red (Ex-Max 502 nm/Em-Max 586 nm)
Alizarin Red is a natural water-soluble pigment derived from Anthraquinones to stain for calcium deposits in tissue and the bone contained in whole mount embryonic specimens. Alizarin Red can be used chromogenically or fluorescently. It has a fluorescence excitation peak at 502 nm and an emission peak at 586nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 502 nm
- Emission maximum
- 586 nm
- Optimal laser line
- 488
- Optimal filter
- 585/42
- Molecular weight
- 342.26
Alizarin Red has an excitation maximum at 502 nm and an emission maximum at 586 nm, a Stokes shift of 84 nm. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is Alizarin Red?
- Alizarin Red is a natural water-soluble pigment derived from Anthraquinones to stain for calcium deposits in tissue and the bone contained in whole mount embryonic specimens. Alizarin Red can be used chromogenically or fluorescently. It has a fluorescence excitation peak at 502 nm and an emission peak at 586nm.
- What laser excites Alizarin Red?
- Alizarin Red is optimally excited by the 488 laser line. Its excitation maximum is 502 nm.
- What filter should I use to detect Alizarin Red?
- A 585/42 filter captures the peak of the Alizarin Red emission near 586 nm with minimal spillover.