Calcein Red-Orange AM
Calcein Red-Orange AM (Ex-Max 573 nm/Em-Max 588 nm)
Calcein Red-Orange, AM, is a fluorogenic red emitting fluorescent molecule (ex 561/ em 573) that penetrates the membranes of live cells. Once inside the live cell, Calcein Red, AM is hydrolyzed by intracellular esterases into the fluorescent Calcein Red which can then be measured via Fluorescence Microscopy, flow cytometer, or fluorescent microplate reader. Reagents that contain an acetoxymethyl ester (AM) or diacetate (DA) side chain, are useful indicators of cell health and vitality, especially when used together with a probe for cell membrane integrity like an impermeant nucleic acid stain or fixable live/dead probe.
Excitation & emission spectra
Key facts
- Excitation maximum
- 573 nm
- Emission maximum
- 588 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
Calcein Red-Orange AM has an excitation maximum at 573 nm and an emission maximum at 588 nm, a Stokes shift of 15 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is Calcein Red-Orange AM?
- Calcein Red-Orange, AM, is a fluorogenic red emitting fluorescent molecule (ex 561/ em 573) that penetrates the membranes of live cells. Once inside the live cell, Calcein Red, AM is hydrolyzed by intracellular esterases into the fluorescent Calcein Red which can then be measured via Fluorescence Microscopy, flow cytometer, or fluorescent microplate reader. Reagents that contain an acetoxymethyl ester (AM) or diacetate (DA) side chain, are useful indicators of cell health and vitality, especially when used together with a probe for cell membrane integrity like an impermeant nucleic acid stain or fixable live/dead probe.
- What laser excites Calcein Red-Orange AM?
- Calcein Red-Orange AM is optimally excited by the 561 laser line. Its excitation maximum is 573 nm.
- What filter should I use to detect Calcein Red-Orange AM?
- A 585/42 filter captures the peak of the Calcein Red-Orange AM emission near 588 nm with minimal spillover.