LipidSpot 488
LipidSpot 488 (Ex-Max 430 nm/Em-Max 586 nm)
Biotium's LipidSpot dyes are used to fluorescently label intracellular lipid droplets in live or fixed cells with no wash step. These dyes remain stable after cells stained with LipidSpot dyes are fixed or permeabilized. LipidSpot 488 has an excitation max at 430 nm and an emission peak at 586 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 430 nm
- Emission maximum
- 586 nm
- Optimal laser line
- 405
- Optimal filter
- 585/42
- Commercial antibodies
- 2 from 1 supplier
LipidSpot 488 has an excitation maximum at 430 nm and an emission maximum at 586 nm, a Stokes shift of 156 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is LipidSpot 488?
- Biotium's LipidSpot dyes are used to fluorescently label intracellular lipid droplets in live or fixed cells with no wash step. These dyes remain stable after cells stained with LipidSpot dyes are fixed or permeabilized. LipidSpot 488 has an excitation max at 430 nm and an emission peak at 586 nm.
- What laser excites LipidSpot 488?
- LipidSpot 488 is optimally excited by the 405 laser line. Its excitation maximum is 430 nm.
- What filter should I use to detect LipidSpot 488?
- A 585/42 filter captures the peak of the LipidSpot 488 emission near 586 nm with minimal spillover.
- How many antibodies are available conjugated to LipidSpot 488?
- FluoroFinder tracks 2 commercially available LipidSpot 488 conjugates, searchable by target, clone, host and application.