mScarlet
mScarlet (Ex-Max 568 nm/Em-Max 589 nm)
mScarlet is an monomeric orange fluorescent protein with excitation and emission maximums of 569 nm and 594 nm, respectively.
Excitation & emission spectra
Key facts
- Excitation maximum
- 568 nm
- Emission maximum
- 589 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
- Extinction coefficient
- 100000
- Quantum yield
- 0.7
mScarlet has an excitation maximum at 568 nm and an emission maximum at 589 nm, a Stokes shift of 21 nm. Its extinction coefficient is 100000 M⁻¹cm⁻¹. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is mScarlet?
- mScarlet is an monomeric orange fluorescent protein with excitation and emission maximums of 569 nm and 594 nm, respectively.
- What laser excites mScarlet?
- mScarlet is optimally excited by the 561 laser line. Its excitation maximum is 568 nm.
- What filter should I use to detect mScarlet?
- A 585/42 filter captures the peak of the mScarlet emission near 589 nm with minimal spillover.