Cy3.5
Cy3.5 (Ex-Max 579 nm/Em-Max 591 nm)
Cy3.5 is an important historical fluorophore from GE Healthcare. It has an excitation peak at 579 nm and an emission peak at 591 nm. Spectrally its similar to Alexa Fluor™ 568.
Excitation & emission spectra
Key facts
- Excitation maximum
- 579 nm
- Emission maximum
- 591 nm
- Optimal laser line
- 561
- Optimal filter
- 585/42
- Relative brightness
- 3 of 5
- Molecular weight
- 608
- Commercial antibodies
- 10 from 4 suppliers
Cy3.5 has an excitation maximum at 579 nm and an emission maximum at 591 nm, a Stokes shift of 12 nm. It is optimally excited by the 561 laser line.
Frequently asked questions
- What is Cy3.5?
- Cy3.5 is an important historical fluorophore from GE Healthcare. It has an excitation peak at 579 nm and an emission peak at 591 nm. Spectrally its similar to Alexa Fluor™ 568.
- What laser excites Cy3.5?
- Cy3.5 is optimally excited by the 561 laser line. Its excitation maximum is 579 nm.
- What filter should I use to detect Cy3.5?
- A 585/42 filter captures the peak of the Cy3.5 emission near 591 nm with minimal spillover.
- How bright is Cy3.5?
- Cy3.5 has a relative brightness of 3 out of 5.
- How many antibodies are available conjugated to Cy3.5?
- FluoroFinder tracks 10 commercially available Cy3.5 conjugates, searchable by target, clone, host and application.