iFluor 514
iFluor® 514 (Ex-Max 525 nm/Em-Max 554 nm)
iFluor® 514 from AAT Bioquest is a fluorophore with an excitation peak at 525 nm and an emission peak at 554 nm. It can be useful in both microscopy and flow cytometry. Spectrally, it is similar to Alexa Fluor™ 514.
Excitation & emission spectra
Key facts
- Excitation maximum
- 525 nm
- Emission maximum
- 554 nm
- Optimal laser line
- 532
- Optimal filter
- 550/40
- Relative brightness
- 1 of 5
iFluor 514 has an excitation maximum at 525 nm and an emission maximum at 554 nm, a Stokes shift of 29 nm. It is optimally excited by the 532 laser line.
Frequently asked questions
- What is iFluor 514?
- iFluor® 514 from AAT Bioquest is a fluorophore with an excitation peak at 525 nm and an emission peak at 554 nm. It can be useful in both microscopy and flow cytometry. Spectrally, it is similar to Alexa Fluor™ 514.
- What laser excites iFluor 514?
- iFluor 514 is optimally excited by the 532 laser line. Its excitation maximum is 525 nm.
- What filter should I use to detect iFluor 514?
- A 550/40 filter captures the peak of the iFluor 514 emission near 554 nm with minimal spillover.
- How bright is iFluor 514?
- iFluor 514 has a relative brightness of 1 out of 5.