Loading the Dye Directory…

Vybrant DyeCycle Orange

Vybrant DyeCycle Orange (Ex-Max 519 nm/Em-Max 653 nm)

Vybrant DyeCycle Orange from Thermo Fisher Scientific is a fluorogenic, cell-permeant nucleic acid stain that can be used to assess cell cycle in live or fixed cells. Vybrant DyeCycle probes are an alternative to the CytoPhase probes from Biolegend. It has an excitation peak at 519 nm and an emission peak at 553 nm.

Excitation & emission spectra

400450500550600650700750800

Key facts

Excitation maximum
519 nm
Emission maximum
553 nm
Optimal laser line
532
Optimal filter
660/20
Commercial antibodies
1 from 1 supplier

Vybrant DyeCycle Orange has an excitation maximum at 519 nm and an emission maximum at 553 nm, a Stokes shift of 34 nm. It is optimally excited by the 532 laser line.

Frequently asked questions

What is Vybrant DyeCycle Orange?
Vybrant DyeCycle Orange from Thermo Fisher Scientific is a fluorogenic, cell-permeant nucleic acid stain that can be used to assess cell cycle in live or fixed cells. Vybrant DyeCycle probes are an alternative to the CytoPhase probes from Biolegend. It has an excitation peak at 519 nm and an emission peak at 553 nm.
What laser excites Vybrant DyeCycle Orange?
Vybrant DyeCycle Orange is optimally excited by the 532 laser line. Its excitation maximum is 519 nm.
What filter should I use to detect Vybrant DyeCycle Orange?
A 660/20 filter captures the peak of the Vybrant DyeCycle Orange emission near 553 nm with minimal spillover.
How many antibodies are available conjugated to Vybrant DyeCycle Orange?
FluoroFinder tracks 1 commercially available Vybrant DyeCycle Orange conjugates, searchable by target, clone, host and application.