About Alexa Fluor 532

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Alexa Fluor™ 532 (AF532, Alexa 532) has an excitation peak at 532 nm and an emission peak at 554 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC, iFluor® 514 (ATT Bioquest) and iFluor® 546 (ATT Bioquest). Many flow cytometers are equipped with 532nm lasers, so while Alexa 532 can be used for flow cytometry, PE is more commonly used due to its brightness and spectral characteristics. This dye is commonly used for fluorescence microscopy applications.

Alternative Dyes to Alexa Fluor 532

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NovaFluor Blue 555 ATTO 532

Dyes similar to Alexa Fluor 532 based on emission

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ATTO 532 ATTO 542 ATTO Rho6G BPE Brilliant Ultraviolet 563 Calcein Orange Cascade Yellow CF405L CF514 CF517ST CF532 CF543 Chromeo 546 CoraFluor-1 CoraFluor-2 CPN550 Yellow Cyanine 3 SE CytoTrack Yellow DY-485XL EFLUXX-ID Gold ER-ID Green iFluor 514 iFluor 532 Janelia Fluor 525 Live/Dead Fix Olive Live/Dead Fix Yellow Live-or-Dye 405/545 Live-or-Dye 510/550 LysoBrite Orange LYSO-ID Green mBanana MemBrite Fix 543/560 MemBrite Fix 543/560 mHoneyDew MitoLite Orange EX405 mKO mKusabira-Orange2 mOrange NovaFluor Blue 555 Nuclear Orange NucSpot 470 NucView 530 Pacific Orange PhenoVue 512 Nucleic Acid Stain – complexed with nucleic acids Pyronin Y Qdot 545 RealBlue 545 Rhodamine 6G VivaFix 398/550 Vybrant DyeCycle Orange ABflo 532 XFD532 CoraFluor 2, amine reactive