About Alexa Fluor 532

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Alexa Fluor® 532 (AF532, Alexa 532) is a yellow-emitting synthetic fluorophore that can be excited by the 532 nm yellow laser and captured with a 575/36 nm bandpass filter. AF532 has an excitation peak at 532 nm and an emission peak at 554 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC, iFluor™ 514 (ATT Bioquest) and iFluor™ 546 (ATT Bioquest). It is uncommon for flow cytometers to be equipped with 532 lasers, so while AF532 can be used for flow cytometry, it is less popular for this application. This dye is commonly used for immunohistochemistry applications and more specifically super-resolution microscopy because it is bright, photostable, and pH insensitive. The Alexa Fluor dye family was originally developed by Molecular Probes, now a part of ThermoFisher Scientific. AlexaFluor is a registered trademark of Invitrogen, ThermoFisher Scientific. Alexa Fluor dyes come in a wide variety of modified formats that allow for easy conjugations or right out of the box use.

Dyes similar to Alexa Fluor 532 based on emission

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Alexa Fluor 430 ATTO 532 ATTO 542 ATTO Rho6G Brilliant Ultraviolet 563 Calcein Orange Cascade Yellow CF405L CF514 CF517ST CF532 CF543 Chromeo 546 CPN550 Yellow Cyanine 3 SE CytoTrack Yellow DY-485XL EFLUXX-ID Gold ER-ID Green iFluor 514 iFluor 532 Janelia Fluor 525 Live/Dead Fix Olive Live/Dead Fix Yellow Live-or-Dye 405/545 Live-or-Dye 510/550 LysoBrite Orange LYSO-ID Green mBanana MemBrite Fix 543/560 mHoneyDew MitoLite Orange EX405 mKO mKusabira-Orange2 mOrange NovaFluor Blue 555 Nuclear Orange NucSpot 470 NucView 530 Pacific Orange Pyronin Y Qdot 545 Rhodamine 6G Spark Blue 550 VivaFix 398/550