532

Laser Line

575/36

Common Filter

532

Excitation Max

554

Emission Max

16,369

Antibodies

1

Conjugation Kits

1

Services


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About Alexa Fluor 532


Alexa Fluor® 532 (AF532, Alexa 532) is a yellow-emitting synthetic fluorophore that can be excited by the 532 nm yellow laser and captured with a 575/36 nm bandpass filter. AF532 has an excitation peak at 532 nm and an emission peak at 554 nm, and is spectrally similar to Cy®3 (GE Healthcare), TRITC, iFluor™ 514 (ATT Bioquest) and iFluor™ 546 (ATT Bioquest). It is uncommon for flow cytometers to be equipped with 532 lasers, so while AF532 can be used for flow cytometry, it is less popular for this application. This dye is commonly used for immunohistochemistry applications and more specifically super-resolution microscopy because it is bright, photostable, and pH insensitive. The Alexa Fluor dye family was originally developed by Molecular Probes, now a part of ThermoFisher Scientific. AlexaFluor is a registered trademark of Invitrogen, ThermoFisher Scientific. Alexa Fluor dyes come in a wide variety of modified formats that allow for easy conjugations or right out of the box use.


Popular Alexa Fluor 532 Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
eBioscience (Thermo Fisher Scientific)
58-0038-41
FC CD3
Human
Mouse
IgG1 κ
UCHT1
25 Tests
$125
View
Thermo Fisher Scientific
58-0259-41
FC CD25
Human
Mouse
IgG1 κ
BC96
25 Tests
$129
View
Thermo Fisher Scientific
58-0149-41
FC CD14
Human
Mouse
IgG1 κ
61D3
25 Tests
$309
View
Thermo Fisher Scientific
58-0038-41
FC CD3
Human
Mouse
IgG1 κ
UCHT1
25 Tests
$309
View
Thermo Fisher Scientific
58-0038-42
FC CD3
Human
Mouse
IgG1 κ
UCHT1
100 Tests
$309
View
Thermo Fisher Scientific
58-0042-82
FC CD4
Mouse
Rat
IgG2a κ
RM4-5
100 μg
$309
View
Thermo Fisher Scientific
58-0451-82
FC CD45
Mouse
Rat
IgG2b κ
30-F11
100 μg
$309
View
Thermo Fisher Scientific
58-0088-42
FC CD8a
Human
Mouse
IgG1 κ
RPA-T8
100 Tests
$309
View
Thermo Fisher Scientific
58-0458-42
FC CD45RA
Human
Mouse
IgG2b κ
HI100
100 Tests
$349
View
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