iFluor 647
iFluor® 647 (Ex-Max 654 nm/Em-Max 670 nm)
iFluor® 647 from AAT Bioquest is a fluorophore with an excitation peak at 654 nm and an emission peak at 670 nm. It can be useful in both microscopy and flow cytometry. Spectrally, it is similar to Alexa Fluor™ 647 and Cy5.
Excitation & emission spectra
Key facts
- Excitation maximum
- 654 nm
- Emission maximum
- 670 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Relative brightness
- 4 of 5
- Extinction coefficient
- 250000
- Quantum yield
- 0.25
- Molecular weight
- 1274.66
- Commercial conjugates
- 131 from 7 suppliers
iFluor 647 has an excitation maximum at 654 nm and an emission maximum at 670 nm, a Stokes shift of 16 nm. Its extinction coefficient is 250000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is iFluor 647?
- iFluor® 647 from AAT Bioquest is a fluorophore with an excitation peak at 654 nm and an emission peak at 670 nm. It can be useful in both microscopy and flow cytometry. Spectrally, it is similar to Alexa Fluor™ 647 and Cy5.
- What laser excites iFluor 647?
- iFluor 647 is optimally excited by the 628-640 laser line. Its excitation maximum is 654 nm.
- What filter should I use to detect iFluor 647?
- A 660/20 filter captures the peak of the iFluor 647 emission near 670 nm with minimal spillover.
- How bright is iFluor 647?
- iFluor 647 has a relative brightness of 4 out of 5 (quantum yield 0.25).
- How many antibodies are available conjugated to iFluor 647?
- FluoroFinder tracks 131 commercially available iFluor 647 conjugates, searchable by target, clone, host and application.