SureLight PerCP
SureLight PerCP (Ex-Max 483 nm/Em-Max 677 nm)
SureLight PerCP from Columbia Biosciences is a fluorescent protein with an excitation peak at 483 nm and an emission peak at 677 nm. SureLight PerCP is available in antibody conjugation kits for use in flow cytometry.
Excitation & emission spectra
Key facts
- Excitation maximum
- 483 nm
- Emission maximum
- 677 nm
- Optimal laser line
- 488
- Optimal filter
- 670/30
- Relative brightness
- 1 of 5
- Extinction coefficient
- 1960000
- Molecular weight
- 35000
- Commercial conjugates
- 7 from 1 supplier
SureLight PerCP has an excitation maximum at 483 nm and an emission maximum at 677 nm, a Stokes shift of 194 nm. Its extinction coefficient is 1960000 M⁻¹cm⁻¹. It is optimally excited by the 488 laser line.
Frequently asked questions
- What is SureLight PerCP?
- SureLight PerCP from Columbia Biosciences is a fluorescent protein with an excitation peak at 483 nm and an emission peak at 677 nm. SureLight PerCP is available in antibody conjugation kits for use in flow cytometry.
- What laser excites SureLight PerCP?
- SureLight PerCP is optimally excited by the 488 laser line. Its excitation maximum is 483 nm.
- What filter should I use to detect SureLight PerCP?
- A 670/30 filter captures the peak of the SureLight PerCP emission near 677 nm with minimal spillover.
- How bright is SureLight PerCP?
- SureLight PerCP has a relative brightness of 1 out of 5.
- How many antibodies are available conjugated to SureLight PerCP?
- FluoroFinder tracks 7 commercially available SureLight PerCP conjugates, searchable by target, clone, host and application.