Janelia Fluor 635
Janelia Fluor® 635 (Ex-Max 635 nm/Em-Max 652 nm)
Janelia Fluor® 635 was developed at the Janelia Campus of the Howard Hughes Medical Institute but is commercialized by other vendors. The Janelia Fluor®s family is unique in that the fluorophores are cell-permeable and are available in photoactivatable forms. These fluorophores were developed for super-resolution microscopy (STED, PALM and STORM) and live-cell microscopy in the HaloTag and SNAP-tag versions. Janelia Fluor® 635 has an excitation peak at 635 nm and an emission peak at 652 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 635 nm
- Emission maximum
- 652 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Relative brightness
- 3 of 5
- Extinction coefficient
- 167000
- Quantum yield
- 0.56
- Commercial antibodies
- 17.8k from 1 supplier
Janelia Fluor 635 has an excitation maximum at 635 nm and an emission maximum at 652 nm, a Stokes shift of 17 nm. Its extinction coefficient is 167000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Janelia Fluor 635?
- Janelia Fluor® 635 was developed at the Janelia Campus of the Howard Hughes Medical Institute but is commercialized by other vendors. The Janelia Fluor®s family is unique in that the fluorophores are cell-permeable and are available in photoactivatable forms. These fluorophores were developed for super-resolution microscopy (STED, PALM and STORM) and live-cell microscopy in the HaloTag and SNAP-tag versions. Janelia Fluor® 635 has an excitation peak at 635 nm and an emission peak at 652 nm.
- What laser excites Janelia Fluor 635?
- Janelia Fluor 635 is optimally excited by the 628-640 laser line. Its excitation maximum is 635 nm.
- What filter should I use to detect Janelia Fluor 635?
- A 660/20 filter captures the peak of the Janelia Fluor 635 emission near 652 nm with minimal spillover.
- How bright is Janelia Fluor 635?
- Janelia Fluor 635 has a relative brightness of 3 out of 5 (quantum yield 0.56).
- How many antibodies are available conjugated to Janelia Fluor 635?
- FluoroFinder tracks 17836 commercially available Janelia Fluor 635 conjugates, searchable by target, clone, host and application.