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SuperBright 645
SuperBright 645 (Ex-Max 414 nm/Em-Max 645 nm)
SuperBright 645 (SB645) is a fluorescent polymer with an excitation peak at 414 nm and an emission peak at 645 nm. SB645 is spectrally similar to StarBright Violet 670 and BV650. This dye is a member of the SuperBright dye family, sold by ThermoFisher Scientific under license from BD Bioscience.
Excitation & emission spectra
Key facts
- Excitation maximum
- 414 nm
- Emission maximum
- 645 nm
- Optimal laser line
- 405
- Optimal filter
- 660/20
- Relative brightness
- 2 of 5
- Commercial antibodies
- 134 from 1 supplier
SuperBright 645 has an excitation maximum at 414 nm and an emission maximum at 645 nm, a Stokes shift of 231 nm. It is optimally excited by the 405 laser line.
Frequently asked questions
- What is SuperBright 645?
- SuperBright 645 (SB645) is a fluorescent polymer with an excitation peak at 414 nm and an emission peak at 645 nm. SB645 is spectrally similar to StarBright Violet 670 and BV650. This dye is a member of the SuperBright dye family, sold by ThermoFisher Scientific under license from BD Bioscience.
- What laser excites SuperBright 645?
- SuperBright 645 is optimally excited by the 405 laser line. Its excitation maximum is 414 nm.
- What filter should I use to detect SuperBright 645?
- A 660/20 filter captures the peak of the SuperBright 645 emission near 645 nm with minimal spillover.
- How bright is SuperBright 645?
- SuperBright 645 has a relative brightness of 2 out of 5.
- How many antibodies are available conjugated to SuperBright 645?
- FluoroFinder tracks 134 commercially available SuperBright 645 conjugates, searchable by target, clone, host and application.