Syto 61
Syto 61 (Ex-Max 615 nm/Em-Max 648 nm)
SYTO 61 from Thermo Fisher Scientific is a cell-permeant, fluorogenic nucleic acid stain that has an excitation peak at 628 nm and an emission peak at 645 nm. SYTO dyes can be used in flow cytometry and fluorescence microscopy.
Excitation & emission spectra
Key facts
- Excitation maximum
- 628 nm
- Emission maximum
- 645 nm
- Optimal laser line
- 628-640
- Optimal filter
- 630/69
Syto 61 has an excitation maximum at 628 nm and an emission maximum at 645 nm, a Stokes shift of 17 nm. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is Syto 61?
- SYTO 61 from Thermo Fisher Scientific is a cell-permeant, fluorogenic nucleic acid stain that has an excitation peak at 628 nm and an emission peak at 645 nm. SYTO dyes can be used in flow cytometry and fluorescence microscopy.
- What laser excites Syto 61?
- Syto 61 is optimally excited by the 628-640 laser line. Its excitation maximum is 628 nm.
- What filter should I use to detect Syto 61?
- A 630/69 filter captures the peak of the Syto 61 emission near 645 nm with minimal spillover.