E2-Crimson
E2-Crimson (Ex-Max 625 nm/Em-Max 650 nm)
E2-Crimson is a tetrameric fluorescent protein derived from the coral anemone Discosoma sp. It has an excitation peak at 611 nm and an emission peak at 646 nm.
Excitation & emission spectra
Key facts
- Excitation maximum
- 611 nm
- Emission maximum
- 646 nm
- Optimal laser line
- 628-640
- Optimal filter
- 660/20
- Extinction coefficient
- 126000
- Quantum yield
- 0.23
E2-Crimson has an excitation maximum at 611 nm and an emission maximum at 646 nm, a Stokes shift of 35 nm. Its extinction coefficient is 126000 M⁻¹cm⁻¹. It is optimally excited by the 628-640 laser line.
Frequently asked questions
- What is E2-Crimson?
- E2-Crimson is a tetrameric fluorescent protein derived from the coral anemone Discosoma sp. It has an excitation peak at 611 nm and an emission peak at 646 nm.
- What laser excites E2-Crimson?
- E2-Crimson is optimally excited by the 628-640 laser line. Its excitation maximum is 611 nm.
- What filter should I use to detect E2-Crimson?
- A 660/20 filter captures the peak of the E2-Crimson emission near 646 nm with minimal spillover.