About 7-AAD

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7-Aminoactinomycin D (7-AAD) is a red-emitting DNA intercalating fluorophore that binds to G-C rich regions of dead cells or cells with compromised membranes. It can be excited by the 488 nm, 532 nm and 561 nm lasers and collected using a 650 nm long-pass filter. 7-AAD has an excitation peak at 546 nm and an emission peak at 647 nm. NucSpot® Far-Red (Biotium) and Sytox AADvanced (Thermo Fisher Scientific) are poplular alternatives to 7-AAD due to reduced spillover characteristics. 7-AAD is primarily used for dead cell staining, as it is excluded from live cells with intact membranes, however due to the dysregulation of plasma membrane charge that happens with apoptosis, 7-AAD often is an indicator of late-stage apoptosis. This dye can be used in Fluorescence Microscopy or Flow Cytometry applications. This is a generic dye that can be found in many supplier's catalogs.

Dyes similar to 7-AAD based on emission

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Alexa Fluor 633 Alexa Fluor 635 ATTO Rho14 BDY 630-X SE BODIPY 630/650 Brilliant Violet 650 Calcein Deep Red CellTracker Deep Red CF620R CF633 CytoTrack Red E2-Crimson eFluor 650NC eFluor 660 Fix Viability eVolve 655 Helix NP NIR iFluor 633 Janelia Fluor 635 LysoBrite NIR MagDot 640 MagDot 655 MITO-ID (Extracellular O2) MitoLite Deep Red FX660 MitoView 633 mNeptune mPlum MultiDot 640 MultiDot 655 Nuclear-ID Red PE-Fire 640 Qdot 655 ReadiLink 633-655 SNARF 1 pH9.0 STAR 635 STAR 635P STAR RED SuperBright 645 Syto 61 Sytox AADvanced Sytox Red TagRFP657 ViaKrome 638