532

Laser Line

670/30

Common Filter

546

Excitation Max

647

Emission Max

21

Antibodies


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About 7-AAD


7-Aminoactinomycin D (7-AAD) is a red-emitting DNA intercalating fluorophore that binds to G-C rich regions of dead cells or cells with compromised membranes. It can be excited by the 488 nm blue laser and collected using a 650 nm long-pass filter. 7-AAD has an excitation peak at 546 nm and an emission peak at 647 nm. NucSpot® Far-Red (Biotium) is a poplular alternative to 7-AAD due to its reduced spillover characteristics. 7-AAD is primarily used for dead cell staining, as it is excluded from live cells with intact membranes. This dye can be used in fluorescent microscopy, imaging, or flow cytometry applications and is particularly useful when performing multicolor experiments due to its large stokes shift. This is a generic dye that can be found in many supplier’s catalogs.


Popular 7-AAD Antibodies


Company/SKU Applications Marker / Reactivity Host / Isotype / Clone Size / Price Details
eBioscience (Thermo Fisher Scientific)
00-6993-50
FC 7-AAD
All Species
N/A
N/A
N/A
2 ml
$45
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BioLegend
420403
FC 7-AAD
All Species
N/A
IgG
200 Tests
$28
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BioLegend
420404
FC 7-AAD
All Species
N/A
IgG
500 Tests
$55
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BD Biosciences
555816
FC 7-AAD
All Species
N/A
N/A
N/A
100 Tests
$70
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BD Biosciences
555815
FC 7-AAD
All Species
N/A
N/A
N/A
500 Tests
$197
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eBioscience (Thermo Fisher Scientific)
00-6993-50
FC 7-AAD
All Species
N/A
N/A
N/A
2 ml
$45
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BD Biosciences
559925
FC 7-AAD
All Species
N/A
N/A
N/A
2 ml
$56
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Bio-Rad
1351102
FC 7-AAD
All Species
N/A
IgG
3 x 3 ml
$286
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Miltenyi Biotec
130-111-568
IF,FC 7-AAD
All Species
N/A
IgG
1 ml
$79
View
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